Alterations of the gut microbiota and metabolomics in children with e-waste lead exposure
Alterations of the gut microbiota and metabolomics in children with e-waste lead exposure
复制标题
电子垃圾铅暴露儿童肠道微生物群和代谢组学的变化
DOI：
10.1016/j.jhazmat.2022.128842
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发表时间：
2022-04-14
影响因子：
13.6
通讯作者：
Huo, Xia
中科院分区：
文献类型：
--
作者：
Zeng, Xiang;Zeng, Zhijun;Huo, Xia
背景：铅暴露可以改变肠道微生物群的组成和代谢物。然而，很少有研究调查儿童的这种关联。方法：从桂屿（电子垃圾拆解区）和浩江（参照区）共招募3 ~ 7岁儿童551名。最终有70名受试者符合纳入标准。采用GFAAS和ICP-MS技术检测血铅和尿铅浓度。采用16s rRNA MiSeq测序技术和气相色谱-质谱（GC-MS）技术分别测定粪便样品中的微生物群和代谢物。结果：贵屿市儿童血、尿铅平均浓度高于好江市。两组间差异属58种，差异代谢物19种，存在广泛而显著的相关性。在微生物群、代谢物和身体发育指标方面，Pb暴露导致两组之间的微生物群、代谢物和身体发育参数差异最为显著。鞘脂代谢和离子转运也可能因铅暴露而改变。结论：暴露于铅与儿童肠道微生物群和代谢组的显著改变有关。需要更多的研究来证实这项研究的结果。
Background: A lead (Pb) exposure can alter the composition and metabolites of gut microbiota. However, few studies investigated this association in the children. Methods: A total of 551 children aged 3-7 years were recruited from Guiyu (the e-waste dismantling area) and Haojiang (the reference area). There were finally 70 subjects met the inclusive criteria. Blood and urinary Pb concentrations were detected by GFAAS and ICP-MS techniques. The microbiota and metabolites were measured in stool samples using 16 S rRNA MiSeq sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively. Results: Average Pb concentrations in the blood and urine of children were higher in Guiyu than in Haojiang. There were 58 kinds of differential genera and 19 types of discrepant metabolites between the two groups, and wide and significant correlations were found between them. Exposure to Pb caused the most significant differences in microbiota, metabolites, and physical development parameters between the two groups in terms of microbiota, metabolites, and physical development indicators. Sphingolipid metabolism and ion transport may also be altered by Pb exposure. Conclusions: Exposure to Pb is associated with significant alterations in the gut microbiota and metabolome in children. More research is needed to confirm the findings of this study.