Distribution of the NisI immunity protein and enhancement of nisin activity by the lipid-free NisI.
Distribution of the NisI immunity protein and enhancement of nisin activity by the lipid-free NisI.
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DOI：
10.1016/s0378-1097(03)00934-0
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发表时间：
2004-02
影响因子：
2.1
通讯作者：
O. Koponen;T. Takala;U. Saarela;M. Qiao;P. Saris
中科院分区：
文献类型：
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作者：
O. Koponen;T. Takala;U. Saarela;M. Qiao;P. Saris
产生抗菌肽Nisin的乳酸乳球菌细胞通过特异性免疫蛋白NisI和NisF/E/G来保护细胞膜。我们在这里表明，大约一半的产生的NisI逃脱脂质修饰（LF-NisI=无脂质NisI），并分泌到培养基中，LF-NisI没有亲和力的细胞ofL。乳酸。测定NisI和乳链菌肽的摩尔比在细胞表面上为约1：10，在培养物上清液中为1：50。纯化的LF-NisI显示出增强乳链菌肽对几种测试的指示菌株的活性。用含有NisFEG转运系统的细胞未观察到LF-NisI对乳链菌肽活性的增强。
Lactococcus lactiscells producing the antibacterial peptide nisin protect their own cytoplasmic membrane by specific immunity proteins, NisI and NisF/E/G. We show here that approximately half of the produced NisI escaped the lipid modification (LF-NisI=lipid-free NisI) and was secreted to the medium, and that LF-NisI had no affinity to cells ofL. lactis. The molar ratio of NisI and nisin was determined to be approximately 1:10 on the cell surface and 1:50 in the culture supernatant. Purified LF-NisI was shown to enhance the activity of nisin against several tested indicator strains. The enhancement of nisin activity by LF-NisI was not observed with cells containing the NisFEG transport system.