Automated apparatus for quantitation of social approach behaviors in mice
Automated apparatus for quantitation of social approach behaviors in mice
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DOI：
10.1111/j.1601-183x.2004.00071.x
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发表时间：
2004-10-01
影响因子：
2.5
通讯作者：
Crawley, JN
中科院分区：
文献类型：
--
作者：
Nadler, JJ;Moy, SS;Crawley, JN
社会功能障碍的小鼠模型，旨在研究社会行为的复杂遗传学，需要一个客观的方法来评分与人类疾病症状相关的社会互动。在这里，我们描述了一个自动化的，三腔室的装置，旨在监测社会互动的鼠标。在每个腔室中花费的时间和进入的次数由检测光电池光束中断的系统自动评分。当用自动化设备测试时，幼年雄性C57 BL/6 J小鼠在含有陌生小鼠的室中比在空室中花费更多的时间（社交性），与观察者评分法获得的结果相似。此外，自动评分检测到的偏好，花更多的时间与一个不熟悉的陌生人比一个更熟悉的同种（偏好社会新奇），类似的观察者得分方法所获得的结果。嗅指向包含陌生小鼠的铁丝笼与在该室中花费的时间显着相关，表明在室中的持续时间代表真正的社会接近行为。房间之间的条目数并没有相关的持续时间在房间里度过的条目，而不是证明了一个有用的控制措施的一般活动。最显著的社会接近行为发生在社交性和社会新奇感偏好测试的前五分钟。这些方法的应用C57 BL/6 J，DBA/2 J和FVB/NJ成年男性显示，所有三个菌株表现出倾向于社交和偏好社会新奇。为了评估陌生小鼠品系对社交性和对社交新奇性的偏好的重要性，C57 BL/6 J受试小鼠与A/J陌生人或C57 BL/6 J陌生人一起测试。社交能力和对社会新奇事物的偏好在两种陌生人中是相似的。自动化设备提供了一种准确和客观的方法来测量小鼠的社会倾向。它的使用可能允许在社交功能障碍的小鼠模型中对小鼠社交行为进行更高通量的评分。
Mouse models of social dysfunction, designed to investigate the complex genetics of social behaviors, require an objective methodology for scoring social interactions relevant to human disease symptoms. Here we describe an automated, three chambered apparatus designed to monitor social interaction in the mouse. Time spent in each chamber and the number of entries are scored automatically by a system detecting photocell beam breaks. When tested with the automated equipment, juvenile male C57BL/6J mice spent more time in a chamber containing a stranger mouse than in an empty chamber (sociability), similar to results obtained by the observer scored method. In addition, automated scoring detected a preference to spend more time with an unfamiliar stranger than a more familiar conspecific (preference for social novelty), similar to results obtained by the observer scored method. Sniffing directed at the wire cage containing the stranger mouse correlated significantly with time spent in that chamber, indicating that duration in a chamber represents true social approach behavior. Number of entries between chambers did not correlate with duration of time spent in the chambers; entries instead proved a useful control measure of general activity. The most significant social approach behavior took place in the first five minutes of both the sociability and preference for social novelty tests. Application of these methods to C57BL/6J, DBA/2J and FVB/NJ adult males revealed that all three strains displayed tendencies for sociability and preference for social novelty. To evaluate the importance of the strain of the stranger mouse on sociability and preference for social novelty, C57BL/6J subject mice were tested either with A/J strangers or with C57BL/6J strangers. Sociability and preference for social novelty were similar with both stranger strains. The automated equipment provides an accurate and objective approach to measuring social tendencies in mice. Its use may allow higher-throughput scoring of mouse social behaviors in mouse models of social dysfunction.