Expression analysis of HMGB1 in histological samples of malignant pleural mesothelioma
Expression analysis of HMGB1 in histological samples of malignant pleural mesothelioma
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DOI：
10.1111/his.13470
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发表时间：
2018-05-01
期刊：
影响因子：
6.4
通讯作者：
Boldorini, Renzo L.
中科院分区：
文献类型：
--
作者：
Rrapaj, Eltjona;Trisolini, Elena;Boldorini, Renzo L.
目的高迁移率族盒1（HMGB 1）是一种染色质结构蛋白，在哺乳动物细胞核中普遍表达。当转运到细胞外时，它作为肿瘤抑制和致癌蛋白。在恶性胸膜间皮瘤（MPM）中，高血清HMGB 1水平与预后不良有关。相反，在MPM tissues.Methods和resultsBiopsy标本170例MPM患者进行了评估，免疫组化和逆转录-聚合酶链反应（RT-PCR），以评估HMGB 1蛋白和基因表达的意义尚不清楚。HMGB 1蛋白表达水平采用半定量系统进行评分，该系统将细胞核、细胞质和两者中阳性染色细胞的强度（0-3）和百分比（0 - 4）相加。最终得分被认为是高（>3）或低（
AimsHigh mobility group box 1 (HMGB1) is a chromatin structural protein, expressed ubiquitously in the nuclei of mammalian cells. When transported extracellularly, it acts as a tumour suppressor and oncogenic protein. In malignant pleural mesothelioma (MPM), high serum levels of HMGB1 have been related to a poor prognosis. Conversely, the significance of HMGB1 expression in MPM tissues is still unclear.Methods and resultsBiopsy samples from 170 patients with MPM were assessed by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) to evaluate HMGB1 protein and gene expression. The expression level of HMGB1 protein was scored using a semiquantitative system that sums the intensity (0-3) and the percentage (from 0 to 4) of positively stained cells in nuclei, cytoplasm and in both. The final score was considered as high (>3) or low (