EXPERIMENTAL DEMONSTRATION OF MULTIPLE-SCATTERING SUPPRESSION IN QUASIELASTIC-LIGHT-SCATTERING SPECTROSCOPY BY HOMODYNE COINCIDENCE TECHNIQUES
EXPERIMENTAL DEMONSTRATION OF MULTIPLE-SCATTERING SUPPRESSION IN QUASIELASTIC-LIGHT-SCATTERING SPECTROSCOPY BY HOMODYNE COINCIDENCE TECHNIQUES
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DOI：
10.1103/physreva.24.1939
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发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
PHILLIES, GDJ
中科院分区：
文献类型：
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作者：
PHILLIES, GDJ
多重散射伪影是准弹性光散射光谱（QELSS）应用中的一个常见难题。一个早期的建议[G。Phillies，J.Chem.Phys.74，260（1981）]的双光束、双检测器光散射光谱仪，它只对单散射光子敏感，在此进行实验测试。发现该装置基本上如预测的那样工作。0.091− μ m聚苯乙烯球在水中的扩散系数与溶质体积分数<$0.01无关。在蛋白质浓度为20重量%时，多重散射对牛血清白蛋白的QELSS光谱没有显著影响。
Multiple-scattering artifacts are a not uncommon difficulty in applications of quasielastic-light-scattering spectroscopy (QELSS). An earlier proposal [G. Phillies, J. Chem. Phys. 74, 260 (1981)] for a two-beam, two-detector light-scattering spectrometer, which is only sensitive to singly scattered photons, is subject here to an experimental test. The device is found to work essentially as predicted. The diffusion coefficient of 0.091− μ m polystyrene spheres in water is concentration independent for solute volume fraction≲ 0.01. Multiple scattering does not have a significant effect on the QELSS spectrum of bovine serum albumin at a protein concentration of 20 wt.%.