Isolation and fluorescence in situ hybridization mapping of 60 cosmid clones on human chromosome 18.
Isolation and fluorescence in situ hybridization mapping of 60 cosmid clones on human chromosome 18.
复制标题
人 18 号染色体上 60 个粘粒克隆的分离和荧光原位杂交作图。
作者：
H. Nakashima;M. Sakai;R. Inaba;T. Imamura
我们已经绘制了60个新的cosestone的短臂和长臂的18号染色体的R-或DAPI显带和同步荧光原位杂交。这些标记物分离自杂交体MS 126 -21，所述杂交体MS 126 -21由保留人18号染色体的人-啮齿动物杂交细胞系制成。22个粘粒探针的地图上的短臂，和31个探针集群在长臂的远侧半带18q21.1和18 q23之间，而其他7个探针被映射到更接近着丝粒周围带18q11.1-q12.3。荧光原位杂交技术已被证明是一种非常有效的基因定位方法。这60个探针将可用于阐明与疾病如四体18 p综合征、18 q-综合征和结肠直肠癌相关的遗传改变。
We have mapped 60 new cosmids on the short and long arms of chromosome 18 either by R- or by DAPI-banding and simultaneous fluorescence in situ hybridization. These markers were isolated from hybrid MS126-21 made from a human-rodent hybrid cell line that retained human chromosome 18. Twenty-two of the cosmid probes map on the short arm, and 31 probes cluster in the distal half of the long arm between bands 18q21.1 and 18q23, while 7 other probes are mapped more proximal to the centromere around bands 18q11.1-q12.3. The technique of fluorescence in situ hybridization has proven to be a very efficient methodology for gene mapping. These 60 probes will be useful in the elucidation of genetic alterations associated with diseases such as tetrasomy 18p syndrome, 18q- syndromes, and colorectal cancer.