Effects of unstirred Disse fluid, nonequilibrium binding, and surface-mediated dissociation on hepatic removal of albumin-bound organic anions.
Effects of unstirred Disse fluid, nonequilibrium binding, and surface-mediated dissociation on hepatic removal of albumin-bound organic anions.
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未搅拌的迪斯液、非平衡结合和表面介导的解离对肝脏去除白蛋白结合有机阴离子的影响。
DOI：
10.1152/ajpgi.1985.248.6.g709
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Luxon,BA
中科院分区：
文献类型：
--
作者：
Forker,EL;Luxon,BA
从观察到与白蛋白结合的有机阴离子具有出人意料的高肝萃取率，我们研究了一个分布式模型，该模型通过调用细胞表面催化白蛋白-阴离子复合体解离的位置来解释这种现象。本报告将该模型扩展到包括结合阴离子到细胞表面的非平衡结合和限速扩散。对扩展模型的模拟分析为解释自由阴离子的表观本征清除提供了明确的依据。该模型与我们用孟加拉玫瑰获得的运输数据完全一致。J.Physiol.248(胃肠测试。肝脏生理素。11)：G702-G708,1985]，但适用于仅估计未知参数向量的选定分量。讨论和说明了另一种方法所固有的不确定性。
Proceeding from the observation that organic anions bound to albumin have hepatic extraction fractions that are unexpectedly high, we have studied a distributed model that accounts for this phenomenon by invoking sites on the cell surface that catalyze the dissociation of albumin-anion complexes. The present report extends this model to include nonequilibrium binding and rate-limiting diffusion of bound anion to the cell surface. Simulation analysis of the extended model provides an unambiguous basis for interpreting the apparent intrinsic clearance of free anion. The model is fully consistent with the transport data that we obtained with rose bengal [Am. J. Physiol. 248 (Gastrointest. Liver Physiol. 11): G702-G708, 1985] but is suited to estimating only selected components of the unknown parameter vector. Uncertainties inherent in an alternate approach are discussed and illustrated.