Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behaviour
Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behaviour
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DOI：
10.1016/s0928-4931(03)00033-x
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发表时间：
2003-06-10
期刊：
影响因子：
--
通讯作者：
Martelet, C
中科院分区：
文献类型：
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作者：
Ponsonnet, L;Reybier, K;Martelet, C
细胞附着和扩散到钛基合金表面是植入技术中的主要参数。在本文中，基质表面疏水性，表面自由能，界面自由能和表面粗糙度进行了研究，以确定这些参数是占主导地位的人成纤维细胞的蔓延。比较了两种测量接触角的方法：座滴法和捕获气泡双探针法。显示了各种工程钛表面（例如商业纯钛（cp-Ti）、钛-铝-钒合金（Ti-6Al-4V）和钛-镍（NiTi））的表面粗糙度与座滴接触角之间的关系。为了消除粗糙度效应，根据基于相同合金的光滑样品上获得的接触角进行表面自由能（SFE）计算。采用Owens-Wendt（OW）和货车Oss（VO）方法和座滴法计算了表面的SFE。OW计算用于获得SFE的色散（gamma（d））和极性（gamma（p））分量，并且VO方法允许达到表面的非极性（gamma（LW））和极性酸碱分量（gamma（ab））。从捕获气泡接触角实验（空气或辛烷气泡在水中），在水中的不同表面的界面自由能得到。发现细胞铺展与SFE的极性成分有关，所有研究表面的界面自由能值均较低，表明此类合金具有良好的生物相容性。(C)2003 Elsevier Science B. V.保留所有权利。
Cell attachment and spreading to titanium-based alloy surfaces is a major parameter in implant technology. In this paper, substratum surface hydrophobicity, surface free energy, interfacial free energy and surface roughness were investigated to ascertain which of these parameters is predominant in human fibroblast spreading. Two methods for contact angle measurement were compared: the sessile drop method and the captive bubble two-probe method. The relationship between surface roughness and the sessile drop contact angles of various engineered titanium surfaces such as commercial pure titanium (cp-Ti), titanium-aluminium-vanadium alloy (Ti-6Al-4V), and titanium-nickel (NiTi), was shown. Surface free energy (SFE) calculations were performed from contact angles obtained on smooth samples based on the same alloys in order to eliminate the roughness effect. SFE of the surfaces have been calculated using the Owens-Wendt (OW) and Van Oss (VO) approaches with the sessile drop method. The OW calculations are used to obtain the dispersive (gamma(d)) and polar (gamma(p)) component of SFE, and the VO approach allows to reach the apolar (gamma(LW)) and the polar acid-base component (gamma(ab)) of the surface. From captive bubble contact angle experiments (air or octane bubble under water), the interfacial free energy of the different surfaces in water was obtained. A relationship between cell spreading and the polar component of SFE was found. Interfacial free energy values were low for all the investigated surfaces indicating good biocompatibility for such alloys. (C) 2003 Elsevier Science B.V. All rights reserved.