Cross-Species Induction of Antimicrobial Compounds, Biosurfactants and Quorum-Sensing Inhibitors in Tropical Marine Epibiotic Bacteria by Pathogens and Biofouling Microorganisms
Cross-Species Induction of Antimicrobial Compounds, Biosurfactants and Quorum-Sensing Inhibitors in Tropical Marine Epibiotic Bacteria by Pathogens and Biofouling Microorganisms
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DOI：
10.1007/s00284-010-9812-1
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发表时间：
2011-03-01
影响因子：
2.6
通讯作者：
Zinjarde, Smita S.
中科院分区：
文献类型：
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作者：
Dusane, Devendra H.;Matkar, Pratiek;Zinjarde, Smita S.
研究了由于跨物种和跨属相互作用而增强或诱导海洋细菌的抗菌、生物表面活性剂和群体感应抑制特性。四种海洋附生细菌（芽孢杆菌 S3、短小芽孢杆菌 S8、地衣芽孢杆菌 D1 和粘质沙雷氏菌 V1）对病原性或生物污损真菌（白色念珠菌 CA 和解脂耶氏酵母 YL）和细菌（铜绿假单胞菌 PA 和短小芽孢杆菌 BP）具有抗菌活性 被选来进行这项研究。当与上述真菌或细菌共培养时，海洋表生细菌表现出抗菌活性、生物表面活性剂产生和群体感应抑制的诱导或增强。针对解脂耶氏酵母 YL 的抗真菌活性是通过病原体或生物污染菌株与海洋芽孢杆菌共培养而诱导的。 S3、短小芽孢杆菌 S8 或地衣芽孢杆菌 D1。对 Ps 的抗菌活性。共培养后，大多数海洋分离株中铜绿假单胞菌 PA 或短小芽孢杆菌 BP 均得到增强。当短小芽孢杆菌 BP 细胞与粘质沙门氏菌 V1、短小芽孢杆菌 S8 或地衣芽孢杆菌 D1 共培养时，生物表面活性剂活性显着增加。当海洋芽孢杆菌菌株时，群体感应抑制指示菌株紫色色杆菌 12472 中的色素减少是明显的。 S3在诱导菌株Ps存在下生长。铜绿假单胞菌 PA，表明群体感应抑制。该研究对于自然环境中的微生物竞争和增强次生代谢产物的产生具有重要的生态和生物技术意义。
Enhancement or induction of antimicrobial, biosurfactant, and quorum-sensing inhibition property in marine bacteria due to cross-species and cross-genera interactions was investigated. Four marine epibiotic bacteria (Bacillus sp. S3, B. pumilus S8, B. licheniformis D1, and Serratia marcescens V1) displaying antimicrobial activity against pathogenic or biofouling fungi (Candida albicans CA and Yarrowia lipolytica YL), and bacteria (Pseudomonas aeruginosa PA and Bacillus pumilus BP) were chosen for this study. The marine epibiotic bacteria when co-cultivated with the aforementioned fungi or bacteria showed induction or enhancement in antimicrobial activity, biosurfactant production, and quorum-sensing inhibition. Antifungal activity against Y. lipolytica YL was induced by co-cultivation of the pathogens or biofouling strains with the marine Bacillus sp. S3, B. pumilus S8, or B. licheniformis D1. Antibacterial activity against Ps. aeruginosa PA or B. pumilus BP was enhanced in most of the marine isolates after co-cultivation. Biosurfactant activity was significantly increased when cells of B. pumilus BP were co-cultivated with S. marcescens V1, B. pumilus S8, or B. licheniformis D1. Pigment reduction in the quorum-sensing inhibition indicator strain Chromobacterium violaceum 12472 was evident when the marine strain of Bacillus sp. S3 was grown in the presence of the inducer strain Ps. aeruginosa PA, suggesting quorum-sensing inhibition. The study has important ecological and biotechnological implications in terms of microbial competition in natural environments and enhancement of secondary metabolite production.