Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation
Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation
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DOI：
10.1038/sj.cr.7290301
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发表时间：
2005-05-01
期刊：
影响因子：
44.1
通讯作者：
Bühring, HJ
中科院分区：
文献类型：
--
作者：
Hennersdorf, F;Florian, S;Bühring, HJ
使用双色流式细胞术，分析了提交给第8届国际人类白细胞分化抗原研讨会（HLDA 8）的> 200种抗体与静息和活化的CD 203 c（+）嗜碱性粒细胞的反应性。四种抗体与静息嗜碱性粒细胞无反应性或弱反应性，与高亲和力IgE受体（Fc β RI）的抗IgE介导的交联激活的嗜碱性粒细胞的反应性增加。这些包括抗CD 164抗体（WS-80160，克隆N6 B6和WS-80162，克隆67 D2），以及两种具有先前未知特异性的试剂，分别鉴定为CD 13（WS-80274，克隆A8）和CD 107 a（WS-80280，克隆E63-880）。活化模式遵循“CD 203 c样”或“CD 63样”活化模式。CD 203 c谱的特征在于（CD 13、CD 164和CD 203 c）的快速且显著的上调，在刺激515分钟后达到最大水平。磷酸肌醇-3-激酶（PI 3 K）特异性抑制剂Wortmannin抑制这些标志物的上调，而1 - 2-O-十四酰基-佛波醇-13-乙酸酯（TPA）在1-2分钟内诱导快速且不依赖于Fc β RI的上调。（CD 63和CD 107 a）仅在20-40分钟后检测到，TPA的上调在60分钟后达到最大水平。总之，我们的数据鉴定了CD 13，CD 107 a，和CD 164作为新的嗜碱性粒细胞活化抗原。基于上调的时间动力学，我们假设“CD 203 c组”和“CD 63组”分子与嗜碱性粒细胞活化的两种不同机制相关。
Using two-colour flow cytometry > 200 antibodies submitted to the 8(th) International Workshop of Human Leukocyte Differentiation Antigens (HLDA8) have been analyzed for their reactivity with resting and activated CD203c(+) basophils. Four antibodies either non-reactive or weakly reactive with resting basophils exhibited an increased reactivity with basophils activated by anti-IgE-mediated cross-linking of the high affinity IgE receptor (Fc epsilon RI). These include antibodies against CD164 (WS-80160, clone N6B6 and WS-80162, clone 67D2), as well as two reagents with previously unknown specificities that were identified as CD13 (WS-80274, clone A8) and CD107a (WS-80280, clone E63-880). The activation patterns followed either the "CD203c-like" or "CD63-like" activation profile. The CD203c profile is characterized by a rapid and significant upregulation (of CD13, CD164, and CD203c), reaching maximum levels after 515 min of stimulation. The Phosphoinositide-3-kinase (PI3K)-specific inhibitor Wortmannin inhibited the upregulation of these markers whereas 12-O-tetradecanoyl-phorbol-13-acetate (TPA) induced a rapid and Fc epsilon RI-independent upregulation within 1-2 min. In the CD63 profile, maximum upregulation (of CD63 and CD107a) was detected only after 20-40 min, and upregulation by TPA reached maximum levels after 60 min. In summary, our data identify CD13, CD107a, and CD 164 as novel basophil-activation antigens. Based on time kinetics of upregulation, we hypothesize that molecules of the "CD203c group" and the "CD63 group" are linked to two different mechanisms of basophil activation.