Identification and characterization of histone lysine methylation modifiers in Fragaria vesca.
Identification and characterization of histone lysine methylation modifiers in Fragaria vesca.
复制标题
草莓中组蛋白赖氨酸甲基化修饰剂的鉴定和表征（正在出版。DOI：10.1038/srep23581）
DOI：
10.1038/srep23581
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发表时间：
2016-04-06
影响因子：
4.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Gu T;Han Y;Huang R;McAvoy RJ;Li Y
二倍体林地草莓(Fragaria Vesca)是一种重要的果树模式，因为它具有基因组小，果实成熟过程不依赖乙烯，果肉来源于花托组织而不是更典型的果树的子房壁等特点。组蛋白甲基化是高等植物基因调控的重要因素，但对其在果实发育中的作用知之甚少。我们已鉴定出45个SET甲基转移酶基因、22个JmjC去甲基酶基因和4个LSD去甲基酶基因。对八种植物中这些组蛋白修饰物的分析支持将这些基因归类为与其功能一致的主要类别。我们还提供了证据表明，通过反转录转座子进行的全基因组复制和分散复制可能在组蛋白修饰基因的扩增中发挥了关键作用。此外，转录组数据表明，一些SET基因的表达随着果实的发育而增加，并在转折期达到高峰。同时，我们观察到这些SET基因的表达对冷热胁迫做出了反应。我们的结果表明，组蛋白甲基化的调控可能在草莓果实发育和对非生物胁迫的反应中发挥关键作用。
The diploid woodland strawberry (Fragaria vesca) is an important model for fruit crops because of several unique characteristics including the small genome size, an ethylene-independent fruit ripening process, and fruit flesh derived from receptacle tissues rather than the ovary wall which is more typical of fruiting plants. Histone methylation is an important factor in gene regulation in higher plants but little is known about its roles in fruit development. We have identified 45 SET methyltransferase, 22 JmjC demethylase and 4 LSD demethylase genes in F. vesca. The analysis of these histone modifiers in eight plant species supports the clustering of those genes into major classes consistent with their functions. We also provide evidence that whole genome duplication and dispersed duplications via retrotransposons may have played pivotal roles in the expansion of histone modifier genes in F. vesca. Furthermore, transcriptome data demonstrated that expression of some SET genes increase as the fruit develops and peaks at the turning stage. Meanwhile, we have observed that expression of those SET genes responds to cold and heat stresses. Our results indicate that regulation of histone methylation may play a critical role in fruit development as well as responses to abiotic stresses in strawberry.