Buffers Affect the Bending Rigidity of Model Lipid Membranes
Buffers Affect the Bending Rigidity of Model Lipid Membranes
复制标题
DOI：
10.1021/la403565f
复制
发表时间：
2014-01-14
期刊：
影响因子：
3.9
通讯作者：
Ipsen, John H.
中科院分区：
文献类型：
--
作者：
Bouvrais, Helene;Duelund, Lars;Ipsen, John H.
在脂质双层的生物物理和生物化学研究中，所使用的缓冲液对膜结构、弹性或物理性质的影响常常被忽略或假定为可忽略不计。然而，我们在这里提出的实验证据，通过弯曲刚度测量上进行的巨大的囊泡，一个更复杂的行为，其中的缓冲分子可能会大大影响磷脂酰胆碱双层的弯曲刚度。此外，在盐和缓冲剂分子的存在下观察到对弯曲模量的协同效应，这对实验者在其研究的数据解释中起到警告作用，因为典型的脂质双层研究含有缓冲剂和离子分子。
In biophysical and biochemical studies of lipid bilayers the influence of the used buffer is often ignored or assumed to be negligible on membrane structure, elasticity, or physical properties. However, we here present experimental evidence, through bending rigidity measurements performed on giant vesicles, of a more complex behavior, where the buffering molecules may considerably affect the bending rigidity of phosphatidylcholine bilayers. Furthermore, a synergistic effect on the bending modulus is observed in the presence of both salt and buffer molecules, which serves as a warning to experimentalists in the data interpretation of their studies, since typical lipid bilayer studies contain buffer and ion molecules.