AUTOPROTEOLYSIS IN HEDGEHOG PROTEIN BIOGENESIS
AUTOPROTEOLYSIS IN HEDGEHOG PROTEIN BIOGENESIS
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DOI：
10.1126/science.7985023
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发表时间：
1994-12-02
期刊：
影响因子：
56.9
通讯作者：
BEACHY, PA
中科院分区：
文献类型：
--
作者：
LEE, JJ;EKKER, SC;BEACHY, PA
由hedgehog（hh）多基因家族编码的细胞外信号蛋白负责脊椎动物和无脊椎动物多种胚胎结构的形成。果蝇hh基因现在已被证明产生两种主要的蛋白质种类，它们是由一个较大的前体的内部自蛋白水解裂解而产生的。在体外降低自身蛋白水解效率的突变减少了体内前体裂解，也损害了HH蛋白的信号传导和图案化活性。这两种HH蛋白具有独特的生化特性和组织分布，这些差异表明了一种机制，可以解释HH在体内的长距离和短距离信号传导活动。
Extracellular signaling proteins encoded by the hedgehog (hh) multigene family are responsible for the patterning of a variety of embryonic structures in vertebrates and invertebrates. The Drosophila hh gene has now been shown to generate two predominant protein species that are derived by an internal autoproteolytic cleavage of a larger precursor. Mutations that reduced the efficiency of autoproteolysis in vitro diminished precursor cleavage in vivo and also impaired the signaling and patterning activities of the HH protein. The two HH protein species exhibited distinctive biochemical properties and tissue distribution, and these differences suggest a mechanism that could account for the long- and short-range signaling activities of HH in vivo.