Regulation of CAPRICE transcription by MYB proteins for root epidermis differentiation in Arabidopsis
Regulation of CAPRICE transcription by MYB proteins for root epidermis differentiation in Arabidopsis
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DOI：
10.1093/pcp/pci096
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发表时间：
2005-06-01
影响因子：
4.9
通讯作者：
Okada, K
中科院分区：
文献类型：
--
作者：
Koshino-Kimura, Y;Wada, T;Okada, K
拟南芥根的表皮细胞分化是了解细胞命运特化的模型系统。两种类型的MYB相关的转录因子参与这种细胞分化。其中之一，CAPRICE（CPC），编码R3型MYB蛋白，是毛细胞分化的正调控因子，并优先在无毛细胞中转录。我们分析了CPC转录的调控机制。CPC启动子的缺失分析表明，无毛细胞特异性转录的CPC基因需要一个69 bp的序列，该区域的串联重复序列足以使其在表皮中表达。该区域包括两个MYB结合位点，当在这些位点引入碱基替换时，CPC的表皮特异性转录被废除。我们通过凝胶迁移率变动实验和酵母单杂交试验表明，WEREWOLF（WER），这是一个R2R3型MYB蛋白，直接结合到这个区域。我们发现，WER也结合到GL2启动子区，表明WER直接调节CPC和GL2的转录通过结合到他们的启动子区。
Epidermal cell differentiation in Arabidopsis root is studied as a model system for understanding cell fate specification. Two types of MYB-related transcription factors are involved in this cell differentiation. One of these, CAPRICE (CPC), encoding an R3-type MYB protein, is a positive regulator of hair cell differentiation and is preferentially transcribed in hairless cells. We analyzed the regulatory mechanism of CPC transcription. Deletion analyses of the CPC promoter revealed that hairless cell-specific transcription of the CPC gene required a 69 bp sequence, and a tandem repeat of this region was sufficient for its expression in epidermis. This region includes two MYB-binding sites, and the epidermis-specific transcription of CPC was abolished when base substitutions were introduced in these sites. We showed by gel mobility shift experiments and by yeast one-hybrid assay that WEREWOLF (WER), which is an R2R3-type MYB protein, directly binds to this region. We showed that WER also binds to the GL2 promoter region, indicating that WER directly regulates CPC and GL2 transcription by binding to their promoter regions.