In vivo immunostimulating activity of the 163-171 peptide of human IL-1 beta.
In vivo immunostimulating activity of the 163-171 peptide of human IL-1 beta.
复制标题
人 IL-1 β 的 163-171 肽的体内免疫刺激活性。
作者：
L. Nencioni;L. Villa;A. Tagliabue;G. Antoni;R. Presentini;F. Perin;S. Silvestri;D. Boraschi
研究了合成的人白细胞介素1 β (IL-1 β)非肽（片段163-171）对T辅助依赖性和T辅助非依赖性抗原的抗体反应的刺激作用。结果表明，非肽增强了用羊红细胞（SRBC）免疫小鼠的脾脏细胞的抗体反应，正如溶血斑块测定所评估的那样。163-171肽对SRBC初次应答的活性呈剂量依赖性，当以100 mg/kg的剂量与抗原一起接种时，其活性最大。此外，163-171肽也能有效增强对SRBC的二次应答。163-171肽的作用是增加抗原特异性细胞的频率，因为在治疗后没有观察到脾脏细胞数量的增加。在所有这些研究中，人重组IL-1 β在质量上的效果与163-171肽相当，最大活性为20 ng/kg。163-171肽和人重组IL-1 β也能够增强对T辅助物不依赖抗原（如SIII）的体内免疫应答，SIII是一种来自III型肺炎链球菌的免疫原性差的多糖抗原。因此，可以提出这种合成的人IL-1 β的非肽可能是用作疫苗佐剂的良好候选物。
The stimulating effect of a synthetic nonapeptide (fragment 163-171) of human interleukin 1 beta (IL-1 beta) on antibody responses to both T helper-dependent and T helper-independent antigens was investigated. It was shown that the nonapeptide enhanced the antibody response, as evaluated in the hemolytic plaque assay, of spleen cells from mice immunized with sheep red blood cells (SRBC). The activity of the 163-171 peptide on the primary response to SRBC was dose-dependent, being maximal when the peptide was inoculated at 100 mg/kg together with the antigen. Moreover, the 163-171 peptide was also effective in enhancing the secondary response to SRBC. The effect of the 163-171 peptide was to augment the frequency of cells specific for the antigen, inasmuch as no increase was ever observed in spleen cell numbers after treatment. In all these studies, human recombinant IL-1 beta gave effects qualitatively comparable to those of the 163-171 peptide, with a maximal activity at 20 ng/kg. Both the 163-171 peptide and human recombinant IL-1 beta were also able to enhance the in vivo immune response to a T helper-independent antigen such as SIII, a poorly immunogenic polysaccharidic antigen from Streptococcus pneumoniae type III. It can therefore be proposed that this synthetic nonapeptide of human IL-1 beta may represent a good candidate for use as adjuvant in vaccines.
影响因子：
2.8
作者：
S. Durum;C. Higuchi;Y. Ron
通讯作者：
S. Durum;C. Higuchi;Y. Ron