A gene with specific and global effects on recombination of sequences from tandemly repeated genes in Saccharomyces cerevisiae.
A gene with specific and global effects on recombination of sequences from tandemly repeated genes in Saccharomyces cerevisiae.
复制标题
对酿酒酵母串联重复基因的序列重组具有特定和全局影响的基因。
DOI：
10.1093/genetics/135.3.711
复制
发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
McWilliams,AD
中科院分区：
文献类型：
--
作者：
Keil,RL;McWilliams,AD
串联重复基因的序列同质性和拷贝数的保存可能需要特定的机制或重组调节。我们已经确定了特异性影响酿酒酵母自然重复重组的突变。 rrm3 突变刺激 rDNA 和铜螯合 (CUP1) 基因自然发生的串联重复中的有丝分裂重组。这种突变不会影响所测试的其他几种类型的重复基因的重组，包括 Ty 元件、交配类型信息和转化产生的重复。除了刺激天然染色体位置处的多个 CUP1 重复序列之间的交换之外，rrm3 还增加了 his4 上存在的 CUP1 单元重复的重组。这表明RRM3基因可能编码一种序列特异性因子，该因子有助于全局抑制可作为串联阵列维持的序列中的有丝分裂交换。
The preservation of sequence homogeneity and copy number of tandemly repeated genes may require specific mechanisms or regulation of recombination. We have identified mutations that specifically affect recombination among natural repetitions in the yeast Saccharomyces cerevisiae. The rrm3 mutation stimulates mitotic recombination in the naturally occurring tandem repeats of the rDNA and copper chelatin (CUP1) genes. This mutation does not affect recombination of several other types of repeated genes tested including Ty elements, mating type information and duplications created by transformation. In addition to stimulating exchange among the multiple CUP1 repeats at their natural chromosomal location, rrm3 also increases recombination of a duplication of CUP1 units present at his4. This suggests that the RRM3 gene may encode a sequence-specific factor that contributes to a global suppression of mitotic exchange in sequences that can be maintained as tandem arrays.