Nucleotide sequence definition of a major human repeated DNA, the Hind III 1.9 kb family.
Nucleotide sequence definition of a major human repeated DNA, the Hind III 1.9 kb family.
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主要人类重复 DNA（Hind III 1.9 kb 家族）的核苷酸序列定义。
DOI：
10.1093/nar/10.10.3211
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发表时间：
1982
影响因子：
14.9
通讯作者：
Manuelidis,L
中科院分区：
文献类型：
--
作者：
Manuelidis,L
分离人 Hind III 1.9 kb 重复 DNA 片段并克隆到 pBR322 中。选择主要与全人类 DNA 消化物中的 1.9 kb Hind III 带杂交的克隆成员进行测序。它是一个 1894 bp 的片段，没有显示出明显的内部重复。序列中观察到很少的 pCG 残基，并且存在大量终止密码子。详细的序列比较证实这是一类新的重复序列，与之前表征的人类卫星 DNA 或 Alu 序列无关。所描述的序列的至少一部分在进化中是保守的。
A human Hind III 1.9 kb repeated DNA fragment was isolated and cloned in pBR322. A cloned member that hybridized predominantly to the 1.9 kb Hind III band in a digest of whole human DNA was chosen for sequencing. It is an 1894 bp fragment that shows no significant internal repeats. Few pCG residues are observed in the sequence and there are numerous stop codons. Detailed sequence comparisons confirm this is a novel class of repeats that is not related to previously characterized human satellite DNAs or Alu sequences. At least a portion of the sequence described is conserved in evolution.