Anti-mutated citrullinated vimentin (anti-MCV) and anti-65 kDa heat shock protein (anti-hsp65): New biomarkers in ankylosing spondylitis
Anti-mutated citrullinated vimentin (anti-MCV) and anti-65 kDa heat shock protein (anti-hsp65): New biomarkers in ankylosing spondylitis
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DOI：
10.1016/j.jbspin.2011.03.010
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Szanto, Sandor
中科院分区：
文献类型：
--
作者：
Bodnar, Nora;Szekanecz, Zoltan;Szanto, Sandor
前言：瓜氨酸化和抗瓜氨酸化蛋白/多肽抗体(ACPA)与类风湿关节炎(RA)的发病机制有关。虽然ACPA是RA的特异和敏感的标志物，但几乎没有关于ACPA治疗强直性脊柱炎(AS)的报道。抗分枝杆菌65 kDa热休克蛋白(Hsp65)抗体在AS中的可能作用尚不清楚。由于AS需要新的实验室生物标志物，我们调查了抗突变的瓜氨酸波形蛋白(MCV)和抗hsp65抗体在AS中的流行情况。方法：43例AS和44例健康对照。血清中抗MCV抗体和抗HSP65抗体分别采用商品化和室内法进行检测。血清自身抗体水平与血沉、C反应蛋白、人类白细胞抗原B27状态、吸烟习惯、疼痛程度、BASDAI、BASFI和Basmi指标相关。结果：AS患者血清抗MCV水平(17.3U/m L，8.3~31.5U/m L)显著高于正常对照组(8.9U/m l，范围5.4-13.3U/m L)(p<；0.01)。43例强直性脊柱炎患者中有16例(37%)抗MCV阳性，44例健康对照中无一例(0%)。AS患者血清中抗HSP65抗体的平均浓度为124.8 AU/mL(范围：27.2~1000AU/mL)，而对照组的抗HSP65抗体水平显著低于对照组(P<0.001)。相关分析显示抗-HSP65抗体阳性(r=0.613；p=0.012)和血清抗-mcv绝对值(r=0.553；p=0.021)均与HSP65抗体水平呈正相关。抗MCV抗体阳性与血沉呈正相关(r=0.437.0 5，P=0.0 3)。结论：抗MCV抗体和抗hsp6 5抗体可能是AS的新生物标志物。(C)2011年，埃尔塞维尔·马森公司代表法国兴业银行出版。
Introduction: Citrullination as well as anti-citrullinated protein/peptide antibodies (ACPA) have been implicated in the pathogenesis of rheumatoid arthritis (RA). While ACPAs are specific and sensitive markers for RA, there have been hardly any reports regarding ACPAs in ankylosing spondylitis (AS). The possible role of antibodies to Mycobacterial 65 kDa heat shock protein (hsp65) has not been characterized in AS. As newlaboratory biomarkers of AS are needed, weinvestigated the prevalence of anti-mutated citrullinated vimentin (MCV) and anti-hsp65 antibodies in AS.Methods: Altogether 43 AS and 44 healthy controls were included in the study. Anti-MCV and anti-hsp65 were determined in sera by commercial and in-house ELISA, respectively. Serum autoantibody levels were correlated with ESR, CRP, HLA-B27 status, smoking habits, pain intensity, BASDAI, BASFI and BASMI indices.Results: Patients with AS had significantly higher serum anti-MCV levels (17.3 U/mL, range: 8.3-31.5 U/mL) in comparison to healthy subjects (8.9 U/mL, range: 5.4-13.3 U/mL) (p < 0.01). Sixteen of the 43 AS patients (37%) and none of the 44 healthy controls (0%) were anti-MCV positive using the cut-off value recommended by the manufacturer (> 20 U/mL). The mean anti-hsp65 concentration in AS sera was 124.8 AU/mL (range: 27.2-1000 AU/mL), while controls exerted significantly lower antihsp65 levels (mean: 51.8 AU/mL; range: 22.5-88.5 AU/mL) (p < 0.001). Correlation analysis revealed that both anti-MCV positivity (r = 0.613; p = 0.012) and absolute serum anti-MCV levels (r = 0.553; p = 0.021) correlated with anti-hsp65 levels. Anti-MCV positivity also correlated with ESR (r = 0.437; p = 0.03).Conclusions: Anti-MCV and anti-hsp65 may be novel biomarkers in AS. (C) 2011 Published by Elsevier Masson SAS on behalf of the Societe Francaise de Rhumatologie.