Characterizing VIP Neurons in the Barrel Cortex of VIPcre/tdTomato Mice Reveals Layer-Specific Differences.
Characterizing VIP Neurons in the Barrel Cortex of VIPcre/tdTomato Mice Reveals Layer-Specific Differences.
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DOI：
10.1093/cercor/bhv202
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Staiger JF
中科院分区：
文献类型：
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作者：
Prönneke A;Scheuer B;Wagener RJ;Möck M;Witte M;Staiger JF
新皮层GABA能中间神经元对皮层回路及其信息处理能力有着深远的影响。抑制性中间神经元的不同亚群可以通过分子标记物来区分，例如小白蛋白、生长抑素和血管活性肠多肽（VIP）。其中，VIP表达的中间神经元引起了极大的兴趣，因为这些神经元似乎在所有主要的新皮层区域中发现的去抑制回路图案。最近的几项研究使用转基因Vip-ires-cre小鼠来特异性靶向VIP表达的中间神经元群体。这使得有必要详细阐明这些动物中VIP神经元Cre表达的敏感性和特异性。因此，我们以层特异性方式定量比较了VIPcre/tdTomato小鼠桶皮质中内源性tdTomato与Vip荧光原位杂交和αVIP免疫组织化学。我们发现，VIPcre/tdTomato小鼠对整个VIP表达神经元群体具有高度敏感性和特异性。在桶皮质中，大约13%的所有GABA能神经元表达VIP。大多数VIP神经元位于II/III层（约60%），而约40%位于桶状皮质的其他层。层II/III VIP神经元显着不同的VIP神经元在层IV-VI在几个形态和膜的特性，这表明层依赖性的功能差异。
Neocortical GABAergic interneurons have a profound impact on cortical circuitry and its information processing capacity. Distinct subgroups of inhibitory interneurons can be distinguished by molecular markers, such as parvalbumin, somatostatin, and vasoactive intestinal polypeptide (VIP). Among these, VIP-expressing interneurons sparked a substantial interest since these neurons seem to operate disinhibitory circuit motifs found in all major neocortical areas. Several of these recent studies used transgenic Vip-ires-cre mice to specifically target the population of VIP-expressing interneurons. This makes it necessary to elucidate in detail the sensitivity and specificity of Cre expression for VIP neurons in these animals. Thus, we quantitatively compared endogenous tdTomato with Vip fluorescence in situ hybridization and αVIP immunohistochemistry in the barrel cortex of VIPcre/tdTomato mice in a layer-specific manner. We show that VIPcre/tdTomato mice are highly sensitive and specific for the entire population of VIP-expressing neurons. In the barrel cortex, approximately 13% of all GABAergic neurons are VIP expressing. Most VIP neurons are found in layer II/III (∼60%), whereas approximately 40% are found in the other layers of the barrel cortex. Layer II/III VIP neurons are significantly different from VIP neurons in layers IV–VI in several morphological and membrane properties, which suggest layer-dependent differences in functionality.