Pregnancy-specific beta 1 glycoprotein mRNA is present in placental as well as non-placental tissues.
Pregnancy-specific beta 1 glycoprotein mRNA is present in placental as well as non-placental tissues.
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妊娠特异性 β1 糖蛋白 mRNA 存在于胎盘和非胎盘组织中。
DOI：
10.1093/oxfordjournals.humrep.a136766
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Cook,RG
中科院分区：
文献类型：
--
作者：
Chan,WY;Tease,LA;Borjigin,J;Chan,PK;Rennert,OM;Srinivasan,B;Shupert,WL;Cook,RG
通过免疫学筛选，从足月胎盘cDNA文库中分离到6个人SP1克隆。所有六个cDNA克隆都交叉杂交。然而，根据插入片段中EcoRI位点的存在与否，至少可以区分两类cDNA。人足月胎盘mRNA的北方印迹分析与所有6个克隆的插入证明存在两种mRNA的1.6和2.4 kb，分别。确定了纯人SP1蛋白的胰蛋白酶消化片段的氨基酸序列，以确认这些cDNA克隆的身份。以其中一个cDNA为探针，从人睾丸cDNA文库和HeLa细胞cDNA文库中分别分离到2个和10个杂交克隆。这些克隆的Southern印迹分析显示，在高严格性下与SP1 cDNA探针的强杂交，表明这些组织中存在高度同源的mRNA种类。
Six human SP1 clones were isolated from a term placental cDNA library by immunological screening. All six cDNA clones cross-hybridized. However, at least two classes of cDNA could be distinguished, based on the presence or absence of anEcoRI site in the insert. Northern blot analysis of human term placental mRNA with all six cloned inserts demonstrated the presence of two mRNA species of 1.6 and 2.4 kb, respectively. The amino acid sequences of tryptic fragments of pure human SP1 protein were determined for confirmation of the identity of these cDNA clones. Using one of the cloned cDNA as probe, two and ten hybridizing clones were isolated from a human testicular cDNA library and a HeLa cell cDNA library, respectively. Southern blot analysis of these clones showed strong hybridization with the SP1 cDNA probe under high stringency, indicating the presence of highly homologous mRNA species in these tissues.