Internal radial perfusion bioreactor promotes decellularization and recellularization of rat uterine tissue
Internal radial perfusion bioreactor promotes decellularization and recellularization of rat uterine tissue
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DOI：
10.1016/j.jbiosc.2021.09.007
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发表时间：
2022-01-13
影响因子：
2.8
通讯作者：
Furukawa, Katsuko S.
中科院分区：
文献类型：
--
作者：
Charoensombut, Narintadeach;Kawabata, Kinyoshi;Furukawa, Katsuko S.
不孕症治疗技术的进步，如体外受精（IVF），帮助许多不孕妇女能够怀孕。然而，这些不孕症的治疗不能适用于患有绝对子宫因素的妇女。利用组织工程技术构建功能性支架，对子宫再生和子宫替代治疗绝对子宫因素性不孕症具有重要意义。在本研究中，我们开发了一个内部径向灌注生物反应器，以促进去细胞和再细胞化的功能性工程子宫组织的制造。结果，与天然组织相比，具有高静水压力随后进行7天内部灌注洗涤的脱细胞子宫组织的DNA含量降低了90%。与天然组织相比，使用内部灌注生物反应器、静态（对照）和高静水压振荡处理的加压子宫组织中的胶原蛋白和蛋白聚糖含量未显示出显著变化。新开发的灌注生物反应器还能够在脱细胞组织中再细胞化，与未接种的细胞组相比，DNA的统计学显著增加614%。波形蛋白和4 '，6-二脒基-2-苯基吲哚（DAPI）在使用生物反应器制造的再细胞化组织中的接种的子宫内膜基质细胞中均匀表达。利用开发的内部径向灌注生物反应器，我们是第一组成功地在所有层（包括上皮、子宫内膜和子宫肌层）中再细胞化子宫组织。这些结果表明，内灌流生物反应器具有潜在的用于制造功能性工程组织，以促进组织再生。(C)2021年，生物技术学会，日本。All rights reserved.
The advances in infertility treatment technologies such as in vitro fertilization (IVF) help many infertile women to be able to get pregnant. However, these infertility treatments cannot be applied to women who are suffering from absolute uterine factor. Fabrication of functional scaffold in tissue engineering approach is believed to play an important role for uterine regeneration and uterus replacement for treating absolute uterine factor infertility. In this research, we developed an internal radial perfusion bioreactor to promote decellularization and recellularization for fabrication of functional engineered uterine tissue. As a result, the DNA contents of the decellularized uterine tissue with high hydrostatic pressure followed by 7 days internal perfusion washing decreased by 90% compared to native tissue. Collagen and proteoglycan contents in the pressurized uterine tissue with the internal perfusion bioreactor, static (control) and shaking treatment with high hydrostatic pressure showed no significant change compared to the native tissue. The newly developed perfusion bioreactor also enabled to recellularize in the decellularized tissue with statistically significant increase of DNA by 614% compared to non-seeded cell groups. Vimentin and 4',6-diamidino-2-phenylindole (DAPI) was homogeneously expressed in the seeded endometrial stromal cells in the recellularized tissue fabricated using the bioreactor. With the developed internal radial perfusion bioreactor, we are the first group to successfully recellularized uterine tissue in all layers including epithelium, endometrium and myometrium. These results showed that the internal perfusion bioreactor has potential to be utilized for fabrication of functional engineered tissue to promote tissue regeneration. (C) 2021, The Society for Biotechnology, Japan. All rights reserved.