EFFECT OF ACYCLOVIR [9-(2-HYDROXYETHOXYMETHYL)GUANINE] ON EPSTEIN-BARR VIRUS-DNA REPLICATION
EFFECT OF ACYCLOVIR [9-(2-HYDROXYETHOXYMETHYL)GUANINE] ON EPSTEIN-BARR VIRUS-DNA REPLICATION
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DOI：
10.1128/jvi.34.2.560-568.1980
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发表时间：
1980-01-01
影响因子：
5.4
通讯作者：
PAGANO, JS
中科院分区：
文献类型：
--
作者：
COLBY, BM;SHAW, JE;PAGANO, JS
报道了阿昔洛韦[9-（2-羟乙氧基甲基）鸟嘌呤]对[人]淋巴母细胞系P3 HR-1和Raji中EB病毒（EBV）DNA复制的影响。浓度为100 μ M的阿昔洛韦完全抑制了超感染的Raji细胞中的EBV DNA合成，但不抑制模拟感染细胞中的DNA合成。病毒产生细胞系P3 HR-1中每个细胞的EBV基因组当量数被阿昔洛韦显著降低; Raji细胞中潜伏的EBV基因组数不受药物影响。在未处理的P3 HR-1培养物上进行的原位细胞杂交显示在15-20%的细胞中存在相对大量的EBV DNA。在100 μ M药物处理后，没有P3 HR-1细胞含有可通过原位细胞杂交检测的EBV DNA水平。间接免疫荧光研究表明，在用100 μ M阿昔洛韦处理7天期间，表达病毒衣壳抗原的P3 HR-1细胞的百分比降低。在用100 μ M无环鸟苷（约14个基因组/细胞）处理后保留在P3 HR-1细胞中的EBV DNA具有共价闭合的环状DNA的性质，平均MW为108 × 10 - 6。106，如通过轮廓长度测量所确定的。
The effect of acyclovir [9-(2-hydroxyethoxymethyl)guanine] on Epstein-Barr virus (EBV) DNA replication in the [human] lymphoblastoid cell lines P3HR-1 and Raji is reported. Acyclovir at a concentration of 100 .mu.M completely inhibited EBV DNA synthesis in superinfected Raji cells but did not inhibit DNA synthesis in mock-infected cells. The number of EBV genome equivalents per cell in the virus-producing cell line P3HR-1 was significantly reduced by acyclovir; the number of latent EBV genomes in Raji cells was not affected by the drug. In situ cytohybridization performed on untreated P3HR-1 cultures revealed the presence of relatively large amounts of EBV DNA in 15-20% of the cells. After a 100 .mu.M drug treatment, no P3HR-1 cells contained levels of EBV DNA detectable by in situ cytohybridization. Indirect immunofluorescence studies demonstrated that during treatment with 100 .mu.M acyclovir for 7 days, the percentage of P3HR-1 cells expressing viral capsid antigen was reduced. The EBV DNA remaining in P3HR-1 cells after treatment with 100 .mu.M acyclovir (approximately 14 genomes/cell) had the properties of covalently closed circular DNA with an average MW of 108 .times. 106 as determined by contour length measurements.