CIRCADIAN PHOTORECEPTION IN THE RETINALLY DEGENERATE MOUSE (RD/RD)
CIRCADIAN PHOTORECEPTION IN THE RETINALLY DEGENERATE MOUSE (RD/RD)
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DOI：
10.1007/bf00198171
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
MENAKER, M
中科院分区：
文献类型：
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作者：
FOSTER, RG;PROVENCIO, I;MENAKER, M
我们研究了光对视网膜退化小鼠（C57 BL/6 J小鼠rd等位基因纯合子：rd/rd）昼夜运动节律的影响。通过改变在昼夜节律时间16给出的15分钟光脉冲（515 nm）的辐照度并测量运动节律的相移的幅度来确定这些小鼠中的昼夜节律光感受的灵敏度。在80日龄的动物上进行实验。尽管RD/RD视网膜中的视觉光感受器丧失，但动物对光线的昼夜节律反应与视网膜正常的小鼠无法区分rd/rd动物的视网膜中未发现光感受器外节（80-100日龄），我们确实鉴定了少量的视锥视蛋白免疫反应性的核周体，而含有视杆细胞的细胞就更少了。使用HPLC，我们证明了视紫红质发色团11-顺式视黄醇的存在和光异构化。rd/rd视网膜含有约2%的11-顺式视黄醇，发现在+/+视网膜。我们还没有确定视蛋白免疫反应性胞体或其他一些未知的细胞类型介导的昼夜光检测rd/rd视网膜。
We have examined the effects of light on circadian locomotor rhythms in retinally degenerate mice (C57BL/6J mice homozygous for the rd allele: rd/rd). The sensitivity of circadian photoreception in these mice was determined by varying the irradiance of a 15 min light pulse (515 nm) given at circadian time 16 and measuring the magnitude of the phase shift of the locomotor rhythm. Experiments were performed on animals 80 days of age. Despite the loss of visual photoreceptors in the rd/rd retina, animals showed circadian responses to light that were indistinguishable from mice with normal retinas (rd/+ and +/+).While no photoreceptor outersegments were identified in the retina of rd/rd animals (80-100 days of age), we did identify a small number of perikarya that were immunoreactive for cone opsins, and even fewer cells that contained rod opsin. Using HPLC, we demonstrated the presence and photoisomerization of the rhodopsin chromophore 11-cis retinaldehyde. The rd/rd retinas contained about 2% of 11-cis retinaldehyde found in +/+ retinas. We have yet to determine whether the opsin immunoreactive perikarya or some other unidentified cell type mediate circadian light detection in the rd/rd retina.