Interactions between Chip and the achaete/scute-daughterless heterodimers are required for Pannier-driven proneural patterning
Interactions between Chip and the achaete/scute-daughterless heterodimers are required for Pannier-driven proneural patterning
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DOI：
10.1016/s1097-2765(05)00079-1
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发表时间：
2000-10-01
期刊：
影响因子：
16
通讯作者：
Heitzler, P
中科院分区：
文献类型：
--
作者：
Ramain, P;Khechumian, R;Heitzler, P
加塔因子Pannier通过增强子结合激活无毛-盾板（ASC）前神经复合体，并为果蝇的感觉刚毛模式提供位置信息。Chip之前被鉴定为背侧选择器Apterous的辅因子，我们在这里表明Apterous和Chip也调节ASC表达。Chip与Pannier合作桥接加塔因子与HLH Ac/Sc和Daughterless蛋白，以允许增强子-启动子相互作用，导致前神经基因的激活，而Apterous拮抗Pannier功能。在Pannier表达域中，Pannier和Apterous可能竞争与其共同的Chip辅因子结合，并且这三种蛋白质之间的准确化学计量对于前神经预型和胸部的区室化都是必不可少的。
The GATA factor Pannier activates the achaete-scute (ASC) proneural complex through enhancer binding and provides positional information for sensory bristle patterning in Drosophila. Chip was previously idientified as a cofactor of the dorsal selector Apterous, and we show here that both Apterous and Chip also regulate ASC expression. Chip cooperates with Pannier in bridging the GATA factor with the HLH Ac/Sc and Daughterless proteins to allow enhancer-promoter interactions, leading to activation of the proneural genes, whereas Apterous antagonizes Pannier function. Within the Pannier domain of expression, Pannier and Apterous may compete for binding to their common Chip cofactor, and the accurate stoichiometry between these three proteins is essential for both proneural prepattern and compartmentalization of the thorax.