Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei.
Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei.
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血流和前循环形式布氏锥虫中动质体 DNA 大环转录本的定位。
DOI：
10.1128/mcb.2.7.845-852.1982
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发表时间：
1982
影响因子：
5.3
通讯作者：
Gelvin,SB
中科院分区：
文献类型：
--
作者：
Stuart,KD;Gelvin,SB
超过80%的布氏锥虫顶质体DNA的大环和大量小环已被克隆。未克隆的大环片段含有很少的限制性核酸内切酶切割位点，不同菌株的大小不同，并且在常规克隆系统中可能不稳定。与血流或前环锥鞭毛体 RNA 制备的 cDNA 与除一个大环片段外的所有片段杂交，但不与小环杂交。在来自血流和前环锥鞭毛体的 RNA 中检测到了 14 个上环转录本。这些转录物的编码序列是本地化的并且占据了大环的大部分。与可变区接壤的大圆的一个区域未发现被转录。我们得出的结论是，大环在血流和前循环锥鞭毛体中大部分但不完全转录，而小环转录在这些阶段很少或不存在。没有观察到可以解释血流和前循环锥鞭毛体之间线粒体呼吸差异的定性转录差异。
Over 80% of the maxicircle and numerous minicircles ofTrypanosoma bruceikinetoplast DNA have been cloned. The uncloned maxicircle segment contains few restriction endonuclease cleavage sites, varies in size among strains, and may be unstable in conventional cloning systems. cDNA prepared to bloodstream or procyclic trypomastigote RNA hybridized to all but one maxicircle segment, but did not hybridize to minicircles. Fourteen maxicircle transcripts were detected in RNA from both bloodstream and procyclic trypomastigotes. The coding sequences for these transcripts were localized and account for most of the maxicircle. One region of the maxicircle, which borders the variable region, was not found to be transcribed. We conclude that the maxicircle is largely but not completely transcribed in both bloodstream and procyclic trypomastigotes, whereas minicircle transcription is minimal or absent in these stages. Qualitative transcriptional differences which could account for mitochondrial respiratory differences between the bloodstream and procyclic trypomastigotes were not observed.