Determination of selected phenothiazines in human plasma by solid-phase extraction and liquid chromatography with coulometric detection
Determination of selected phenothiazines in human plasma by solid-phase extraction and liquid chromatography with coulometric detection
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DOI：
10.1016/j.aca.2008.06.040
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发表时间：
2008-08-29
影响因子：
6.2
通讯作者：
Raggi, Maria Augusta
中科院分区：
文献类型：
--
作者：
Saracino, Maria Addolorata;Amore, Mario;Raggi, Maria Augusta
本文建立了一种新的基于液相色谱-库仑检测器的吩噻嗪类药物（氯丙嗪、丙嗪、氟奋乃静和左美丙嗪）血药浓度测定方法。在Discovery五氟苯基丙基柱上，使用由乙腈（32%）和pH 1.9磷酸盐缓冲液（68%）组成的移动的流动相分离药物。异丙嗪用作内标。在氧化电位为+0.500V的条件下进行了检测。采用氰丙基柱固相萃取，建立了一种新的净化方法，对所有分析物都有良好的萃取率，绝对回收率大于91.0%。检测器响应在氯丙嗪、丙嗪和左美丙嗪的血浆浓度范围为0.5-250.0 ng mL（-1），氟奋乃静的血浆浓度范围为0.2-4.0 ng mL（-1）内呈线性。以日内和日间相对标准偏差值表示的精密度结果良好，均低于3.9%。该方法已成功地应用于接受吩噻嗪类药物治疗的精神病患者的血药浓度分析。(c)2008 Elsevier B. V.保留所有权利。
A new analytical method, based on liquid chromatography with coulometric detection, has been developed and applied to the determination of selected phenothiazines (chlorpromazine, promazine, fluphenazine and levomepromazine) in human plasma. The drugs were separated on a Discovery pentafluorophenylpropyl column, using a mobile phase composed of acetonitrile (32%) and a pH 1.9 phosphate buffer (68%). Promethazine was used as the internal standard. Detection was carried out at an oxidation potential of +0.500V A novel clean-up procedure was developed by means of solid-phase extraction, using cyanopropyl cartridges, which gave good extraction yield for all the analytes, with absolute recovery values higher than 91.0%. The detector response was linear over a plasma concentration range of 0.5-250.0 ng mL(-1) for chlorpromazine, promazine and levomepromazine and of 0.2-4.0 ng mL(-1) for fluphenazine. Precision results, expressed by the intra-day and the inter-day relative standard deviation values, were good, being lower than 3.9%. Accuracy data were satisfactory as well.The method has been successfully applied to the analysis of drug plasma levels of psychiatric patients undergoing therapy with selected phenothiazines. (c) 2008 Elsevier B.V. All rights reserved.