The Ionotropic Receptors IR21a and IR25a mediate cool sensing in Drosophila
The Ionotropic Receptors IR21a and IR25a mediate cool sensing in Drosophila
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DOI：
10.7554/elife.13254
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发表时间：
2016-04-29
期刊：
影响因子：
7.7
通讯作者：
Garrity, Paul A.
中科院分区：
文献类型：
--
作者：
Ni, Lina;Klein, Mason;Garrity, Paul A.
动物依靠高度敏感的温度感受器来寻找最佳温度，但热传感的分子机制尚不清楚。果蝇幼虫的背器官冷细胞（DOCC）是一组对热敏感的神经元，对于幼虫避免冷至关重要。在这里，我们证明 DOCC 冷感是由离子型受体 (IR) 介导的，IR 是无脊椎动物化学传感领域广泛研究的感觉受体家族。我们发现两个 IR，IR21a 和 IR25a，是调节 DOCC 对冷却的反应所必需的，也是避免冷却行为所必需的。此外，我们发现 IR21a 的异位表达可以以 Ir25a 依赖性方式赋予冷响应性，这表明 IR21a 在热传感中具有指导作用。总之，这些数据表明 IR 家族受体可以共同发挥作用，介导极其敏感的热感觉。
Animals rely on highly sensitive thermoreceptors to seek out optimal temperatures, but the molecular mechanisms of thermosensing are not well understood. The Dorsal Organ Cool Cells (DOCCs) of the Drosophila larva are a set of exceptionally thermosensitive neurons critical for larval cool avoidance. Here, we show that DOCC cool-sensing is mediated by Ionotropic Receptors (IRs), a family of sensory receptors widely studied in invertebrate chemical sensing. We find that two IRs, IR21a and IR25a, are required to mediate DOCC responses to cooling and are required for cool avoidance behavior. Furthermore, we find that ectopic expression of IR21a can confer cool-responsiveness in an Ir25a-dependent manner, suggesting an instructive role for IR21a in thermosensing. Together, these data show that IR family receptors can function together to mediate thermosensation of exquisite sensitivity.