Mass Spectrometry Imaging: An Expeditious and Powerful Technique for Fast in Situ Lignin Assessment in Eucalyptus
Mass Spectrometry Imaging: An Expeditious and Powerful Technique for Fast in Situ Lignin Assessment in Eucalyptus
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DOI：
10.1021/ac500220r
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发表时间：
2014-04-01
影响因子：
7.4
通讯作者：
Mazzafera, Paulo
中科院分区：
文献类型：
--
作者：
Araujo, Pedro;Ferreira, Monica Siqueira;Mazzafera, Paulo
植物生物质被认为是生产生物乙醇的一种替代方法。在发酵过程中，植物生物量无法将纤维素转化为更简单的碳水化合物，这在一定程度上是由于木质素，但用于分析木质素成分的标准方法经常使用有毒溶剂，而且费力和耗时。桉树是世界上主要的纤维素来源，利用MS成像技术对该属的木质素进行了研究。用二氧化硅覆盖两种桉树的手工切割切片，用基质辅助激光吸附电离(MALDI)-成像质谱(MS)直接分析。利用文献中可获得的有关可溶木素亚基和结构的信息来追踪它们在切片中的分布，并使用软件图像进行相对定量。MALDI成像分析中常用的基质不能很好地用于植物材料的分析，取而代之的是薄层色谱(TLC)级的硅胶。共检测到22种化合物，并对其进行了相对定量。还有可能确定丁香基和愈创木基单木醇之间的比例，这是每个物种的特征。由于样品的制备方法简单，本文提出的MALDI成像方法可以在常规分析中取代复杂而繁琐的MS方法来研究木质素的组成。
Plant biomass has been suggested as an alternative to produce bioethanol. The recalcitrance of plant biomass to convert cellulose into simpler carbohydrates used in the fermentation process is partially due to lignin, but the standard methods used to analyze lignin composition frequently use toxic solvents and are laborious and time-consuming. MS imaging was used to study lignin in Eucalyptus, since this genus is the main source of cellulose in the world. Hand-cut sections of stems of two Eucalyptus species were covered with silica and directly analyzed by matrix-assisted laser sesorption ionization (MALDI)-imaging mass spectrometry (MS). Information available in the literature about soluble lignin subunits and structures were used to trace their distribution in the sections and using a software image a relative quantification could be made. Matrixes routinely used in MALDI-imaging analysis are not satisfactory to analyze plant material and were efficiently substituted by thin layer chromatography (TLC) grade silica. A total of 22 compounds were detected and relatively quantified. It was also possible to establish a proportion between syringyl and guaiacyl monolignols, characteristic for each species. Because of the simple way that samples are prepared, the MALDI-imaging approach presented here can replace, in routine analysis, complex and laborious MS methods in the study of lignin composition.