Identification of anther-specific genes in a cruciferous model plant, Arabidopsis thaliana, by using a combination of Arabidopsis macroarray and mRNA derived from Brassica oleracea
Identification of anther-specific genes in a cruciferous model plant, Arabidopsis thaliana, by using a combination of Arabidopsis macroarray and mRNA derived from Brassica oleracea
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DOI：
10.1007/s00497-002-0159-z
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发表时间：
2003-01-01
影响因子：
--
通讯作者：
Watanabe, M
中科院分区：
文献类型：
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作者：
Amagai, M;Ariizumi, T;Watanabe, M
拟南芥是十字花科植物的一员，是模式植物，其基因组是第一个被测序的高等植物基因组。由于花的体积较小，很难解剖和分离不同发育阶段的生殖器官(花药和雌蕊)。为了在全基因组范围内鉴定拟南芥的花药特异基因，将拟南芥的cDNA微阵列与另一种十字花科植物甘蓝的花药和雌蕊的cdna杂交。通过扫描每个克隆的信号强度，并进行聚类分析，鉴定出52个花药特异基因。这些克隆包含几个已被鉴定的花药特异基因，以及新的花药特异基因。在对拟南芥和甘蓝的mRNA进行RT-PCR分析时，有1/3的克隆的表达模式与基因芯片测定的结果相似。该异源杂交分析体系(拟南芥cDNA微阵列与油菜组织特异性mRNAs)同样适用于其他模式物种及其近缘种。
Arabidopsis thaliana, a member of the Brassicaceae, is a model plant whose genome was the first higher plant genome to be sequenced. Because of the small size of the flowers, it is difficult to dissect and separate reproductive organs (anthers and pistils) at different developmental stages in A. thaliana. In order to perform genome-wide identification of anther-specific genes in A. thaliana, an Arabidopsis cDNA macroarray was hybridized to cDNA derived from anthers and pistils of another crucifer, Brassica oleracea. After scanning the signal intensity for each clone, and cluster analysis, 52 anther-specific genes were identified. These clones contained several anther-specific genes that have already been characterized, as well as novel anther-specific genes. In RT-PCR analysis with mRNA of A. thaliana and B. oleracea, the expression pattern of one-third of the clones was similar to that determined by cDNA macroarray. This system of heterologous hybridization analysis (Arabidopsis cDNA macroarray vs Brassica tissue-specific mRNA) should be applicable to other model species and their close relatives.