Protective effect of glutamine-enriched early enteral nutrition on intestinal mucosal barrier injury after liver transplantation in rats
Protective effect of glutamine-enriched early enteral nutrition on intestinal mucosal barrier injury after liver transplantation in rats
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富含谷氨酰胺的早期肠内营养对大鼠肝移植后肠黏膜屏障损伤的保护作用
DOI：
10.1016/j.amjsurg.2008.11.039
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发表时间：
2010-01-01
影响因子：
3
通讯作者：
Gong, Jian-Ping
中科院分区：
文献类型：
--
作者：
Li, Yang;Chen, Yong;Gong, Jian-Ping
背景技术背景：谷氨酰胺强化的早期肠内营养（Gln EEN）对肝移植（LT）后肠黏膜屏障损伤的保护作用尚不明确。观察肠黏膜形态学变化，检测肠黏膜丙二醛（malondialdehyde，MDA）、分泌型免疫球蛋白（secretory immunoglobulin，sIgA）、血浆内毒素、D-乳酸、血清肿瘤坏死因子-α（tumor necrosis factor-alpha，TNF-α）、细菌移位率、肠核因子-κ B、TNF-α和细胞间粘附分子-1的表达。在移植后12、24和48小时，肠道sIgA水平降低;丙二醛、内毒素水平降低。D-乳酸、TNF-α、细菌移位率、肠核因子-κ B、TNF-α和细胞间粘附分子-1的表达均增加。然而，在接受Gln-EEN治疗的受者中，前面提到的参数的变化在24至48 hours.CONCLUSIONS：Our data show that Gln-EEN is a potent protectant against intestinal mucosal barrier injury after LT.（C）2010 Elsevier Inc. All rights reserved.
BACKGROUND: The effect of glutamine-enriched early enteral nutrition (Gln-EEN) on intestinal mucosal barrier injury after liver transplantation (LT) remains uncertain.METHODS: The Wistar-to-Wistar rat LT model was used to explore the protective effect of Gln-EEN. Morphologic changes of intestinal mucosa, levels of intestinal malondialdehyde and secretory immunoglobulin (sIgA), plasma endotoxin, D-lactic acid, serum tumor necrosis factor-alpha (TNF-alpha), rates of bacterial translocation, and expression of intestinal nuclear factor-kappa B, TNF-alpha, and intercellular adhesion molecule-1 were determined.RESULTS: After LT, intestinal mucosa was damaged seriously. At 12, 24, and 48 hours post-transplantation, levels of intestinal sIgA were decreased; levels of malondialdehyde, endotoxin. D-lactic acid, and TNF-alpha, the ratio of bacterial translocation, and the expression of intestinal nuclear factor-kappa B, TNF-alpha, and intercellular adhesion molecule-1 all were increased. However, changes in earlier-mentioned parameters in recipients treated with Gln-EEN were attenuated remarkably at 24 to 48 hours.CONCLUSIONS: Our data show that Gln-EEN is a potent protectant against intestinal mucosal barrier injury after LT. (C) 2010 Elsevier Inc. All rights reserved.