Chalcone synthase EaCHS1 from Eupatorium adenophorum functions in salt stress tolerance in tobacco
Chalcone synthase EaCHS1 from Eupatorium adenophorum functions in salt stress tolerance in tobacco
复制标题
紫茎泽兰查尔酮合酶 EaCHS1 在烟草耐盐胁迫中发挥作用
DOI：
10.1007/s00299-015-1751-7
复制
发表时间：
2015-05-01
影响因子：
6.2
通讯作者：
Cheng Hongmei
中科院分区：
文献类型：
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作者：
Chen Lijuan;Guo Huiming;Cheng Hongmei
EaCHS1通过维持活性氧稳态在植物耐盐胁迫中发挥作用。查耳酮合成酶（CHS）是类黄酮生物合成的关键酶。CHS的表达受广泛的环境刺激，包括紫外线、病原体攻击和生物钟。然而，关于CHS与盐胁迫之间的关系的研究却很少。本研究分别构建了EaCHS1的过表达载体和RNA干扰载体，并将其转入烟草中。EaCHS1的过表达增加了苯丙烷途径中下游类黄酮的产生和相关基因的表达。它还提高了种子萌发和根发育过程中对盐胁迫的抗性。与此相反，异源沉默烟草内源CHS的保守EaCHS1片段有相反的效果。总之，我们的研究结果表明，改变EaCHS1在植物中的表达水平，改变类黄酮的积累，并通过维持ROS的稳态来调节植物对盐胁迫的耐受性。
EaCHS1 functions in the tolerance of plantlets to salinity stress by maintaining ROS homeostasis. Chalcone synthase (CHS) is an essential enzyme in the biosynthesis of flavonoids. Expression of CHS is governed by a wide range of environmental stimuli, including UV light, pathogen attack, and circadian clocks. However, little research exists on the relationship between CHS and salinity stress. In this work, we constructed separate overexpression and RNA interference vectors of EaCHS1, and transferred them into tobacco. Overexpression of EaCHS1 increased the production of downstream flavonoids and the expressions of related genes in the phenylpropanoid pathway. It also improved resistance to salinity stress during seed germination and root development. In contrast, heterologous silencing of endogenous CHS in tobacco by a conserved EaCHS1 fragment had opposite effect. Together, our results indicated that changing the expression level of EaCHS1 in plants alters the accumulation of flavonoids and regulates plantlet tolerance to salinity stress by maintaining ROS homeostasis.