A SURVEY OF MEMBRANE PEPTIDASES IN 2 HUMAN COLONIC CELL-LINES, CACO-2 AND HT-29
A SURVEY OF MEMBRANE PEPTIDASES IN 2 HUMAN COLONIC CELL-LINES, CACO-2 AND HT-29
复制标题
DOI：
10.1042/bj2840595
复制
发表时间：
1992-06-01
影响因子：
4.1
通讯作者：
TURNER, AJ
中科院分区：
文献类型：
--
作者：
HOWELL, S;KENNY, AJ;TURNER, AJ
在两个人结肠癌细胞系中检测细胞表面肽酶的表达。Caco-2和HT-29酶测定揭示了Caco-2细胞系（传代次数82-88）上存在8种细胞表面肽酶，即氨肽酶N。二肽基肽酶IV，肽基二肽酶A（血管紧张素转换酶）。氨肽酶P、氨肽酶W、内肽酶-24.11、γ-谷氨酰转肽酶和膜二肽酶。二肽基肽酶IV和内肽酶-24.11的存在下，也证实了免疫化学。培养15 d后，Caco-2细胞的氨肽酶P、肽基二肽酶A和碱性磷酸酶活性达到平台期，膜二肽酶活性开始下降。相反。氨基肽酶N、二肽基肽酶IV和内肽酶-24.11活性在培养26天后仍在上升。发现传代数181-183的Caco-2细胞缺乏内肽酶-24.11，但维持了二肽基肽酶IV的表达。研究了两个HT-29细胞群。标准的未分化群体和分化群体都仅表达三种肽酶：二肽基肽酶IV、氨肽酶W和羧肽酶M。在分化的HT-29细胞中，二肽基肽酶IV的活性在14-21天后开始趋于稳定，而氨肽酶W的活性仍在上升，羧肽酶M的活性已开始下降。在这组细胞表面肽酶中观察到的活性谱的这些差异表明这些细胞系，特别是Caco-2，是研究其表达调控的有用模型。
The expression of cell-surface peptidases was examined in two human colon carcinoma cell lines. Caco-2 and HT-29. Enzymic assays revealed the presence of eight cell-surface peptidases on a Caco-2 cell line (passage number 82-88), namely aminopeptidase N. dipeptidyl peptidase IV, peptidyl dipeptidase A (angiotension-converting enzyme). aminopeptidase P. aminopeptidase W, endopeptidase-24.11, gamma-glutamyl transpeptidase and membrane dipeptidase. The presence of dipeptidyl peptidase IV and endopeptidase-24.11 was also confirmed immunochemically. After 15 days culture, the activities of aminopeptidase P. peptidyl dipeptidase A and alkaline phosphatase activities on Caco-2 cells reached a plateau, and that of membrane dipeptidase began to decline. In contrast. aminopeptidase N, dipeptidyl peptidase IV and endopeptidase-24.11 activities were still rising after 26 days in culture. Caco-2 cells of passage number 181-183 were found to lack endopeptidase-24.11, but maintained dipeptidyl peptidase IV expression. Two populations of HT-29 cells were surveyed. Both the standard, undifferentiated population and a differentiated population expressed only three peptidases: dipeptidyl peptidase IV, aminopeptidase W and carboxypeptidase M. In the differentiated HT-29 cells the activity of dipeptidyl peptidase IV after 14-21 days was beginning to plateau whereas aminopeptidase W activity was still rising and that of carboxypeptidase M had begun to decline. These differences in activity profiles observed among this group of cell-surface peptidases indicate that these cell lines, especially Caco-2, are useful models to study the regulation of their expression.