Growth factor deposition in anterior subcapsular cataract
Growth factor deposition in anterior subcapsular cataract
复制标题
DOI：
10.1016/j.jcrs.2004.11.039
复制
发表时间：
2005-06-01
影响因子：
2.8
通讯作者：
Ohnishi, Y
中科院分区：
文献类型：
--
作者：
Ishida, I;Saika, S;Ohnishi, Y
目的：应用免疫组织化学方法检测生长因子和细胞外基质(ECM)在前囊下白内障(ASC)中的分布，探讨生长因子在ASC发生发展中的作用。结果：1、V、VI型胶原蛋白、纤维连接蛋白、纤维蛋白-1和潜伏的转化生长因子结合蛋白-1(LTBP-1)定位于ASC组织中的ECM。IV型胶原定位于细胞外基质和囊膜。晶状体上皮细胞表达α-平滑肌肌动蛋白(α-SMA)。晶状体上皮细胞和ECM染色均为转化生长因子β2和转化生长因子β3，但部分标本中可检测到转化生长因子β1潜伏期相关肽。成纤维细胞生长因子-2(FGF-2)和肝细胞生长因子-α(HGF-α)定位于ECM。结论：ASC中存在基质成分(胶原类型、纤维连接蛋白、纤维蛋白-1)和生长因子，如生长因子1-LAP、转化生长因子β2、转化生长因子β3和肝细胞生长因子-α。纤维蛋白-1可能是转化生长因子βS的储存库。这些生长因子可能调节晶状体上皮细胞的表型改变和行为。ASC的晶状体上皮细胞中MAP激动级联和转化生长因子β信号均被激活。
Purpose: To characterize immunohistochemically the distribution of growth factors and extracellular matrix (ECM) components in an anterior subcapsular cataract (ASC) and to determine the role of growth factors in the development of ASC.Setting: Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan.Methods: During cataract surgery in 22 patients, anterior capsules with an ASC were obtained. Sections of each specimen were immunostained with a panel of antibodies against ECM components, growth factors, cytoskeletal components, and signal transduction-related molecules.Results: Collagen types 1, V, and VI; fibronectin; fibrillin-1; and latent transforming growth factor 0 binding protein-1 (LTBP-1) were localized to the ECM in ASC tissues. Collagen IV was localized to the ECM and the capsule. Lens epithelial cells (LECs) were positive for a-smooth muscle actin (alpha SMA). Lens epithelial cells and ECM stained for transforming growth factor beta 2 (TGF beta 2) and TGF beta 3 in all samples, but TGF beta 1 latency-associated peptide (TGF beta 1-LAP) were detected in some samples. Fibroblast growth factor-2 (FGF-2) and hepatocyte growth factor-alpha (HGF-alpha) were localized to the ECM. Lens epithelial cells with nuclear staining for Erk-1, the mitogen-activated protein kinase (MAP kinase) cascade-related molecule, and Smad3, 1 of the Smad family members involving TGF beta signaling, were detected.Conclusions: Matrix components (ie, collagen types, fibronectin, fibrillin-1), as well as growth factors such as TGF beta 1-LAP, TGF beta 2, TGF beta 3, FGF-2, and HGF-alpha, were detected in ASC. Fibrillin-1 might serve as a repository for TGF beta s. These growth factors may modulate the phenotypic alteration and behavior of LECs. The MAP kinase cascade and TGF beta signaling are both activated in LECs in ASC.