A catalogue of resistance gene homologs and a chromosome-scale reference sequence support resistance gene mapping in winter wheat
A catalogue of resistance gene homologs and a chromosome-scale reference sequence support resistance gene mapping in winter wheat
复制标题
抗性基因同源物目录和染色体规模参考序列支持冬小麦抗性基因图谱
DOI：
10.1101/2022.01.26.477808
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Kale S
中科院分区：
文献类型：
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作者：
Kale S
抗性基因图谱是育种者对抗不断进化的病原体的武器库中不可或缺的组成部分。借助高通量测序，即使在具有大型多倍体基因组的作物物种中，也可以组装抗性基因目录。在此，我们报告了 907 个冬小麦基因型多样性组中抗性基因同源物的捕获测序和组装，其中包括异地基因库种质和当前的优良品种。此外，我们使用精确的长读长测序和染色体构象捕获测序来构建cv的染色体规模基因组序列组装。 Attraktion，欧洲冬小麦的代表优良品种。我们通过以下方式说明我们的资源对育种者和遗传学家的价值：(i) 比较植物遗传资源和优良品种中的抗性基因补充；(ii) 使用基于参考和无参考的 GWAS 方法对真菌病黄锈病和叶锈病进行全基因组关联扫描 (GWAS)。在 cv 的组装中仔细检查了 GWAS 峰下的基因内容。吸引力。
A resistance gene atlas is an integral component of the breeder’s arsenal in the fight against evolving pathogens. Thanks to high‐throughput sequencing, catalogues of resistance genes can be assembled even in crop species with large and polyploid genomes. Here, we report on capture sequencing and assembly of resistance gene homologs in a diversity panel of 907 winter wheat genotypes comprisingex situgenebank accessions and current elite cultivars. In addition, we use accurate long‐read sequencing and chromosome conformation capture sequencing to construct a chromosome‐scale genome sequence assembly of cv. Attraktion, an elite variety representative of European winter wheat. We illustrate the value of our resource for breeders and geneticists by (i) comparing the resistance gene complements in plant genetic resources and elite varieties and (ii) conducting genome‐wide associations scans (GWAS) for the fungal diseases yellow rust and leaf rust using reference‐based and reference‐free GWAS approaches. The gene content under GWAS peaks was scrutinized in the assembly of cv. Attraktion.