Human pancreatic carcinoma cells are sensitive to photodynamic therapy in vitro and in vivo
Human pancreatic carcinoma cells are sensitive to photodynamic therapy in vitro and in vivo
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DOI：
10.1046/j.1365-2168.1999.01132.x
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发表时间：
1999-07-01
影响因子：
9.6
通讯作者：
Aprahamian, M
中科院分区：
文献类型：
--
作者：
Hajri, A;Coffy, S;Aprahamian, M
工作背景：本研究的目的是评估光动力疗法（PDT）对人胰腺癌细胞在体外和在animal model.Methods的效率：人胰腺癌细胞系提交给PDT与脱镁叶绿酸（Phn），叶绿素衍生物，在培养和移植到无胸腺小鼠后。用5 J/cm（2）能量处理的培养基（10（-10）-10（-5）mol/l）和用100 J/cm（2）PDT治疗的荷瘤裸鼠（30 mg/kg腹腔注射）测试Ph a。PDT的效果进行了评估，在体外使用增殖，凋亡和克隆形成试验和在体内对肿瘤生长和肿瘤necrosis.Results的诱导：PDT抑制肿瘤细胞的生长在文化中影响DNA的完整性。这种肿瘤细胞光损伤在低浓度（10（-7）mol/l）下开始，如克隆形成和肿瘤生长试验所证实的。结论：PDT在低剂量、弱能量照射下可破坏胰腺癌细胞。它通过温和的方案诱导细胞凋亡以及更强的方案诱导细胞凋亡和/或坏死来发挥这种杀瘤作用。
Background: The aim of this study was to assess the efficiency of photodynamic therapy (PDT) on human pancreatic cancer cells in vitro and in an animal model.Methods: Human pancreatic tumour cell lines were submitted to PDT with pheophorbide a (Phn), a chlorophyll derivative, in culture and after grafting into athymic mice. Ph a was tested in culture (10(-10)-10(-5) mol/l) with a 5-J/cm(2) energy treatment and on tumour-bearing Nude mice (30 mg/kg intraperitoneally) with a 100-J/cm(2) PDT session. The effect of PDT was assessed in vitro using proliferative, apoptotic and clonogenic tests and in vivo on tumour growth and on the induction of tumour necrosis.Results: PDT inhibited tumour cell growth in culture by affecting DNA integrity. This tumour cell photodamage started at low concentration (10(-7)mol/l) as corroborated by clonogenic and tumour growth tests. A strong necrosis was achieved in vivo with a single PDT session.Conclusion: PDT destroyed human pancreatic carcinoma after low photosensitizer supply and weak energy application. It exerted this tumoricidal effect via apoptosis induction with a gentle protocol, and apoptosis and/or necrosis with a stronger protocol.