Transient gene expression in Medicago truncatula leaves via Agroinfiltration.
Transient gene expression in Medicago truncatula leaves via Agroinfiltration.
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通过农杆菌渗透在蒺藜苜蓿叶中瞬时基因表达。
DOI：
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发表时间：
2013
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通讯作者：
R. Macknight
中科院分区：
文献类型：
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作者：
Kelsey L. Picard;Robyn Lee;R. Hellens;R. Macknight
瞬时表达是基因功能表征的有效方法。在本章中，我们概述了使用根癌农杆菌渗透在蒺藜苜蓿叶中瞬时表达构建体的方案。使用定量实时 PCR，我们证明含有豆类花青素生成 1 (LAP1) 转录因子的构建体的渗透会导致关键生物合成基因的强烈上调，并在短短 3 天后导致叶子中花青素的积累。因此，该方法为发现蒺藜蒺藜转录因子下游靶标提供了一种快速而有力的方法。
Transient expression is a powerful method for the functional characterization of genes. In this chapter, we outline a protocol for the transient expression of constructs in Medicago truncatula leaves using Agrobacterium tumefaciens infiltration. Using quantitative real-time PCR we demonstrate that the infiltration of a construct containing the LEGUME ANTHOCYANIN PRODUCTION 1 (LAP1) transcription factor results in the strong upregulation of key biosynthetic genes and the accumulation of anthocyanin pigment in the leaves after just 3 days. Thus, this method provides a rapid and powerful way to the discovery of downstream targets of M. truncatula transcription factors.