PAR-1 is required for morphogenesis of the Caenorhabditis elegans vulva
PAR-1 is required for morphogenesis of the Caenorhabditis elegans vulva
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DOI：
10.1006/dbio.2002.0866
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发表时间：
2003-01-01
影响因子：
2.7
通讯作者：
Kemphues, KJ
中科院分区：
文献类型：
--
作者：
Hurd, D;Kemphues, KJ
秀丽隐杆线虫外阴为后生动物发育过程中模式形成和器官形态发生的遗传分析提供了一个简单的模型。我们已经发现极性蛋白PAR-1在外阴发育中的重要作用。胚胎后PAR-1的RNA干扰导致外阴突出表型。我们发现，在外阴发育过程中消耗PAR-1对命运规范或前体增殖没有可检测到的影响，但似乎特异性地改变了形态发生。使用根尖连接相关的GFP标记，我们发现PAR-1缺失导致外阴的两个镜像对称的半部分连接成一个单一的、连贯的器官失败。通常形成外阴腹侧环的细胞不能相互接触或粘附，从而形成不完整的环状体，而背环则呈现不同的异常形态。我们还发现PAR-1在形态发生过程中经历了从根尖连接到基底外侧结构域的再分配。尽管已知PAR-1在细胞极性中的作用，但我们没有观察到上皮细胞极性各种标记物分布的可检测差异。我们认为，在外阴形态发生过程中，细胞皮层的PAR-1活性对于介导细胞形状变化、细胞表面组成或细胞信号传导至关重要。(C) 2003 Elsevier Science（美国）
The Caenorhabditis elegans vulva provides a simple model for the genetic analysis of pattern formation and organ morphogenesis during metazoan development. We have discovered an essential role for the polarity protein PAR-1 in the development of the vulva. Postembryonic RNA interference of PAR-1 causes a protruding vulva phenotype. We found that depleting PAR-1 during the development of the vulva has no detectable effect on fate specification or precursor proliferation, but instead seems to specifically alter morphogenesis. Using an apical junction-associated GFP marker, we discovered that PAR-1 depletion causes a failure of the two mirror-symmetric halves of the vulva to join into a single, coherent organ. The cells that normally form the ventral vulval rings fail to make contact or adhere and consequently form incomplete toroids, and dorsal rings adopt variably abnormal morphologies. We also found that PAR-1 undergoes a redistribution from apical junctions to basolateral domains during morphogenesis. Despite a known role for PAR-1 in cell polarity, we have observed no detectable differences in the distribution of various markers of epithelial cell polarity. We propose that PAR-1 activity at the cell cortex is critical for mediating cell shape changes, cell surface composition, or cell signaling during vulval morphogenesis. (C) 2003 Elsevier Science (USA).