SUPERSATURATION IN SICKLE-CELL HEMOGLOBIN SOLUTIONS
SUPERSATURATION IN SICKLE-CELL HEMOGLOBIN SOLUTIONS
复制标题
DOI：
10.1073/pnas.73.9.3035
复制
发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
EATON, WA
中科院分区：
文献类型：
--
作者：
HOFRICHTER, J;ROSS, PD;EATON, WA
动力学抑制的Hb S的凝胶化相比，在Hb S的溶解度的变化，当溶解度被改变CO，pH值或尿素。通过一种新的技术，在同一样品上测量了延迟时间和凝胶化程度。延迟时间td与Hb S溶解度的高次幂（30-40）成比例。连同先前报道的浓度依赖性，该结果表明，该速率是成比例的高功率的过饱和度，S，定义为总血红蛋白S浓度的平衡溶解度的比率。结果符合过饱和方程td-1 = γ Sn，其中γ是经验常数（约10-7 s-1），n约为35。过饱和方程可以成功地解释镰状细胞动力学的观察结果，并用于估计镰状细胞病患者产生显著临床获益所需的镰状细胞延迟时间的增加。
The kinetic inhibition of the gelation of Hb S is compared to the change in Hb S solubility, when the solubility is altered by CO, pH or urea. By means of a new technique, the delay time and the extent of gelation are measured on the same sample. The delay time, td, is proportional to a high power (30-40) of the Hb S solubility. Together with the previously reported concentration dependence, this result demonstrates that the rate is proportional to a high power of the supersaturation, S, defined as the ratio of the total Hb S concentration to the equilibrium solubility. The results obey the supersaturation equation td-1 = .gamma.Sn, where .gamma. is an empirical constant (about 10-7 s-1) and n is about 35. The supersaturation equation can successfully account for observations on the kinetics of cell sickling and is used to estimate the increase in the delay time for sickling necessary to produce significant clinical benefit to patients with sickle cell disease.