Annotation and characterization of Cd-responsive metal transporter genes in rapeseed (Brassica napus)
Annotation and characterization of Cd-responsive metal transporter genes in rapeseed (Brassica napus)
复制标题
油菜中镉响应金属转运蛋白基因的注释和表征
DOI：
10.1007/s10534-017-0072-4
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发表时间：
2018-02
期刊：
影响因子：
3.5
通讯作者：
Yang ZM
中科院分区：
文献类型：
--
作者：
Zhang XD;Meng JG;Zhao KX;Chen X;Yang ZM
在高等植物中，重金属转运体负责金属的吸收、转运和动态平衡。这些金属包括必需金属，如锌(锌)或锰(锰)，以及非必需金属，如镉(镉)或铅(铅)。虽然在模式植物(如拟南芥和水稻)中发现了一些重金属转运体，但对它们在油菜(Brassica Napus)中的功能知之甚少。油菜是一种重要的油料作物，是世界上第三大植物油来源。重要的是，油菜由于其巨大的干重生产力和中到高的Cd积累，长期以来一直被认为是植物修复的理想候选者。在这项研究中，270个金属转运蛋白基因(MTG)来自B.利用生物信息学和高通量测序对油菜基因组进行了鉴定和注释。大多数mtg(74.8%，202/270)通过对苗期文库的RNA测序(RNA-seq)得到验证。根据序列同源性，将其分为YSL、OPT、NRAMP、COPT、ZIP、CDF/MTP、HMA、MRP和PDR等9个超家族。RNA测序分析了来自B的202个非冗余mtg。其中有108个mtg基因在Cd胁迫下差异表达，62个基因在Cd胁迫下显著诱导表达。这些差异表达基因(Deg)分散在油菜基因组中。QRT-PCR对部分基因进行了验证。Mtgs onb的基因组分布分析。油菜染色体的进化扩展可能是通过等位基因的局部复制实现的。
In higher plants, heavy metal transporters are responsible for metal uptake, translocation and homeostasis. These metals include essential metals such as zinc (Zn) or manganese (Mn) and non-essential metals like cadmium (Cd) or lead (Pb). Although a few heavy metal transporters have been well identified in model plants (e.g. Arabidopsis and rice), little is known about their functionality in rapeseed (Brassica napus).B. napusis an important oil crop ranking the third largest sources of vegetable oil over the world. Importantly,B. napushas long been considered as a desirable candidate for phytoremediation owning to its massive dry weight productivity and moderate to high Cd accumulation. In this study, 270 metal transporter genes (MTGs) fromB. napusgenome were identified and annotated using bioinformatics and high-throughput sequencing. Most of the MTGs (74.8%, 202/270) were validated by RNA-sequencing (RNA-seq) the seedling libraries. Based on the sequence identity, nine superfamilies including YSL, OPT, NRAMP, COPT, ZIP, CDF/MTP, HMA, MRP and PDR have been classified. RNA-sequencing profiled 202 non-redundant MTGs fromB. napusseedlings, of which, 108 MTGs were differentially expressed and 62 genes were significantly induced under Cd stress. These differentially expressed genes (DEGs) are dispersed in the rapeseed genome. Some of the genes were well confirmed by qRT-PCR. Analysis of the genomic distribution of MTGs onB. napuschromosomes revealed that their evolutional expansion was probably through localized allele duplications.
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影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
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作者：
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影响因子：
3.5
作者：
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通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
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