Determination of nitrocatecholamines in rat brain using high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection
Determination of nitrocatecholamines in rat brain using high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection
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DOI：
10.1016/j.chroma.2007.07.005
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发表时间：
2007-09-14
影响因子：
4.1
通讯作者：
Funatsu, Takashi
中科院分区：
文献类型：
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作者：
Tsunoda, Makoto;Uchino, Erika;Funatsu, Takashi
建立了用高效液相色谱-过氧草酸盐化学发光（POCL）反应检测法测定大鼠脑内硝基儿茶酚胺（nitroCAs: nitroNE）、nitroepinephrine （nitroE）和nitro多巴胺（nitroDA）的方法。该方法包括在线提取胺类，包括硝基rocas，然后在ODS柱上分离，用还原剂柱还原硝基rocas，乙二胺衍生化，最后进行POCL反应检测。减速柱的使用使硝基有机磷农药的检测灵敏度提高了45 ~ 172%。检测限（信噪比为3）分别为：nitroNE 75 fmol， nitroE 125 fmol, nitroDA 150 fmol。用该方法测定了大鼠脑组织中硝基酮的含量，同时测定了mtroE和nitroDA的含量。大鼠脑组织中mtroNE和nitroE的浓度分别为9.2 +/- 2.0和13.5 +/- 2.5 pmol/g组织（n = 5）。(c) 2007 Elsevier B.V.。
We have developed a method to determine nitrocatecholamines (nitroCAs: nitronorepinephrine (nitroNE), nitroepinephrine (nitroE), and nitrodopamine (nitroDA)) in rat brain using high-performance liquid chromatography-peroxyoxalate chemiluminescence (POCL) reaction detection. This method consists of in-line extraction of amines, including nitroCAs, followed by separation on an ODS column, reduction of nitroCAs with a reducer column, derivatization with ethylenediamine, and finally the POCL reaction detection. Use of the reducer column increased the sensitivity of detection of nitroCAs by 45-172%. The limits of detection (signal-to-noise ratio of 3) were 75 fmol for nitroNE, 125 fmol for nitroE, and 150 fmol for nitroDA. By using this method, not only nitroNE, but mtroE and nitroDA were determined in rat brain. The concentrations of mtroNE and nitroE in rat brain were 9.2 +/- 2.0 and 13.5 +/- 2.5 pmol/g tissue, respectively (n = 5). (c) 2007 Elsevier B.V. All rights reserved.