Proteomic analysis of 2-monochloropropanediol (2-MCPD) and 2-MCPD dipalmitate toxicity in rat kidney and liver in a 28-days study.
Proteomic analysis of 2-monochloropropanediol (2-MCPD) and 2-MCPD dipalmitate toxicity in rat kidney and liver in a 28-days study.
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在一项为期 28 天的研究中，对 2-一氯丙二醇 (2-MCPD) 和 2-MCPD 二棕榈酸酯对大鼠肾脏和肝脏的毒性进行了蛋白质组学分析。
DOI：
10.1016/j.fct.2018.08.013
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
A. Lampen
中科院分区：
文献类型：
--
作者：
F. Frenzel;A. Oberemm;A. Braeuning;A. Lampen
2-一氯丙二醇 (2-MCPD)、3-一氯丙二醇 (3-MCPD) 及其脂肪酸酯最近被确定为含脂肪和含盐食品中的热致污染物。 3-MCPD 的毒性先前已被详细研究过。糖酵解和细胞氧化还原功能的紊乱似乎与 3-MCPD 毒性有关。相比之下，2-MCPD 或 2-MCPD 酯的毒理学数据非常少，特别是在分子水平上。因此，本研究旨在全面概述 2-MCPD 和 2-MCPD 二棕榈酸酯（一种代表性的 2-MCPD 脂肪酸酯）在大鼠肾脏和肝脏中诱导的蛋白质组变化。在雄性大鼠体内进行的为期 28 天的体内强饲口服毒性研究中，使用亚毒剂量 10mg/kg 体重的 2-MCPD 或等摩尔剂量的 2-MCPD 二棕榈酸酯。使用每个治疗组 5 只动物的材料进行二维凝胶电泳和质谱蛋白质鉴定，并结合生物信息学数据挖掘，以获得有关观察到的蛋白质组变化的分子基础的信息。获得的数据表明 2-MCPD 暴露对肾脏的毒性后果，并提供证据表明 2-MCPD 通过与 3-MCPD 不同的机制在大鼠肾脏中发挥其细胞作用。
2-monochloropropanediol (2-MCPD), 3-monochloropropanediol (3-MCPD) and their fatty acid esters have recently been identified as heat-induced contaminants in fat- and salt-containing foodstuff. Toxicity of 3-MCPD has been studied previously in some detail. Disturbance of glycolysis and cellular redox functions appear to be involved in 3-MCPD toxicity. By contrast, only very few toxicological data are available for 2-MCPD or 2-MCPD esters, especially at the molecular level. This study was therefore aimed to provide a comprehensive overview of proteomic alterations induced in rat kidney and liver by 2-MCPD and 2-MCPD dipalmitate, a representative 2-MCPD fatty acid ester. Sub-toxic doses of 10 mg/kg body weight 2-MCPD, or equimolar doses of 2-MCPD dipalmitate were applied in a 28-day in vivo gavage oral toxicity study in male rats. Two-dimensional gel electrophoresis and mass-spectrometric protein identification using material from 5 animals per treatment group were employed together with bioinformatic data mining to obtain information about the molecular basis of the observed proteomic alterations. Obtained data indicate toxic consequences of 2-MCPD exposure in the kidney and provide evidence that 2-MCPD exerts its cellular effects in rat kidney by mechanisms different from 3-MCPD.
影响因子：
3.4
作者：
Steiner SR;Milton E;Philbert MA
通讯作者：
Philbert MA