Decreased expression of LASS2 is associated with worse prognosis in meningiomas
Decreased expression of LASS2 is associated with worse prognosis in meningiomas
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DOI：
10.1007/s11060-014-1441-2
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发表时间：
2014-06-01
影响因子：
3.9
通讯作者：
Xiong, Ji
中科院分区：
文献类型：
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作者：
Ke, Rong-Hu;Wang, Yin;Xiong, Ji
酵母LAG 1（LASS 2）的人类长寿保证同源物2已被证明在各种肿瘤中具有关键作用。在这项研究中，我们的目的是评估LASS 2表达水平的预后意义，并将其与脑膜瘤患者常用的生物标志物：Ki-67，p53和孕激素受体（PR）进行比较。首先，分别采用定量PCR和免疫组化（IHC）方法检测50例新鲜脑膜瘤组织和143例石蜡包埋脑膜瘤组织中LASS 2的表达。随后，LASS 2免疫染色评价其临床意义。此外，评估了LASS 2表达与常见生物标志物的相关性。PCR和免疫组化结果均显示LASS 2在高级别脑膜瘤中的表达明显低于I级脑膜瘤和正常脑组织（P均< 0.01）。免疫组化结果显示LASS 2强度分布（ID）评分与肿瘤大小、脑浸润深度、肿瘤复发及临床病程显著相关（均P <0.0 1），而与性别及Simpson分级无相关性。此外，较低的LASS 2 ID评分与较短的总生存期和无进展生存期显著相关（P < 0.01）。Pearson分析显示ID评分与Ki-67和p53显著相关，而与PR无关，多因素分析显示LASS 2是独立的预后因素（P < 0.05）。据我们所知，这是第一次研究LASS 2的表达，并确定其作为脑膜瘤预后的潜在生物标志物。
Homo sapiens longevity assurance homolog 2 of yeast LAG1 (LASS2) has been indicated to have a critical role in various tumors. In the study, we aimed to evaluate the LASS2 expression level in prognostic significance and compare it with commonly used biomarkers: Ki-67, p53 and progesterone receptor (PR) for patients with meningiomas. Firstly, 50 fresh tissues and 143 paraffin-embedded meningiomas samples were analyzed for LASS2 expression by quantitative PCR and immunohistochemistry (IHC), respectively. Subsequently, LASS2 immunostaining was evaluated for its clinical significance. Furthermore, Correlations of LASS2 expression with common biomarkers were assessed. Both PCR and IHC results showed LASS2 was downregulated in high-grade meningiomas in comparison with that of grade I or normal brain (all P < 0.01). IHC results demonstrated LASS2 intensity distribution (ID) score was significantly correlated with tumor size, brain invasion, tumor recurrence and clinical course (all P < 0.01), whereas no correlation of LASS2 ID score with sex or Simpson grade. Moreover, lower LASS2 ID score was strikingly associated with shorter overall and progression-free survival (P < 0.01). Pearson's analysis revealed the ID score was significantly reversely associated with Ki-67 and p53 but not with PR. More importantly,multivariate analyses revealed that LASS2 was an independent prognostic factor (P < 0.05). To our knowledge, it is the first time to investigate the expression of LASS2 and identify it as a potential biomarker for prognosis in meningiomas.