Age-dependent thickening of glomerular basement membrane has no major effect on glomerular hydraulic conductivity.
Age-dependent thickening of glomerular basement membrane has no major effect on glomerular hydraulic conductivity.
复制标题
年龄依赖性肾小球基底膜增厚对肾小球水力传导率没有重大影响。
DOI：
10.1093/ndt/gfh067
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
H. Schurek
中科院分区：
文献类型：
--
作者：
K. Neumann;C. Kellner;K. Kühn;H. Stolte;H. Schurek
背景
用2月龄和18月龄Wistar大鼠研究了肾小球基底膜(GBM)增厚对肾小球毛细血管壁有效水力传导度(K)的影响。
方法
采用微穿刺法，测定了无细胞灌流肾脏皮质肾小球的超滤特性。由于该制剂中的过滤分数很低(3%)，这是由于在与体内条件相当的肾小球过滤速率下的高灌注率，所以在整个过滤表面上提供了均匀的超滤条件。在一定的灌流压力下固定后，在先前研究的肾小球的光镜切片上获得肾小球毛细血管(S)的表面。
结果
肾小球超滤系数(K(F))青年大鼠为0.025 nL/s·mm Hg，老年大鼠为0.038 nL/s mm Hg(P<；0.0005)，S青年大鼠为0.140 mm(2)，老年大鼠为0.244 mm(2)(P<；0.0005)。然而，尽管根据电子显微镜切片估计基底膜厚度增加了2.4倍，但k没有显著差异(青年大鼠为18.0nL/s.mm Hg.cm(2)，老年大鼠为15.8nL/s.mm Hg.cm(2))。
结论
这些发现表明，大鼠肾小球基底膜厚度的增厚并未显著增加肾小球毛细血管壁的水力阻力。
BACKGROUND
The effect of the increasing thickness of the glomerular basement membrane (GBM), which is seen in ageing rats, on the effective hydraulic conductivity (k) of the glomerular capillary wall was studied in Wistar rats aged 2 and 18 months.
METHODS
With the use of micropuncture techniques, ultrafiltration characteristics of cortical glomeruli were determined in isolated cell-free perfused kidneys. Because the filtration fraction in this preparation is low (3%) as a consequence of high perfusion rates at glomerular filtration rates comparable with in vivo conditions, uniform ultrafiltration conditions are provided over the whole filtering surface. After fixation at a defined perfusion pressure, the surface of glomerular capillaries (S) was obtained morphometrically on light microscopic sections of the glomeruli studied previously.
RESULTS
The glomerular ultrafiltration coefficient (K(f)) was 0.025 nl/s.mmHg in young rats and 0.038 nl/s.mmHg in old rats (P<0.0005) and S was 0.140 mm(2) in young and 0.244 mm(2) in old rats (P<0.0005). However, k was not significantly different (18.0 nl/s.mmHg.cm(2) in young and 15.8 nl/s.mmHg.cm(2) in old rats) despite a 2.4-fold increase of GBM thickness as estimated from electron microscopic sections.
CONCLUSIONS
These findings indicate that the age-dependent increase of GBM thickness in rat kidneys did not substantially increase hydraulic resistance of the glomerular capillary wall.
DOI：
--
发表时间：
1999-11
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
K. Tryggvason
通讯作者：
K. Tryggvason
水和蛋白质的渗透性由肾小球上皮裂隙隔膜调节。
DOI：
10.1681/asn.v4121957
发表时间：
1994
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
Blantz,RC;Gabbai,FB;Peterson,O;Wilson,CB;Kihara,I;Kawachi,H;Shimizu,F;Yamamoto,T
通讯作者：
Yamamoto,T