Effects of prostaglandins on hamster seminal vesicle responses to catecholamines and acetylcholine.
Effects of prostaglandins on hamster seminal vesicle responses to catecholamines and acetylcholine.
复制标题
前列腺素对仓鼠精囊对儿茶酚胺和乙酰胆碱反应的影响。
DOI：
10.1093/biolreprod/6.2.224
复制
发表时间：
1972
影响因子：
3.6
通讯作者：
P. Stahl
中科院分区：
文献类型：
--
作者：
P. L. Risley;P. Stahl
比较前列腺素E1、E2、A1、Fia对肾上腺素、去甲肾上腺素和乙酰胆碱刺激下仓鼠精囊平滑肌收缩反应的影响。仓鼠精囊为比较这些药理激动剂的生理作用提供了合适的制剂。来自同一只动物的成对精囊在肌肉浴准备中同步反应，并用于这些测试。对这些刺激物的反应增强发生在所有前列腺素存在的情况下。以atg/ml估计增强剂的相对有效性。肾上腺素和去甲肾上腺素的E1、E2、A1和Fia的剂量比分别约为1:2:5:10。在乙酰胆碱刺激下，PGE2与PGE1在电位憎恨反应中同样有效。这些结果近似并证实了Naimzada（1969）关于这些pg对豚鼠精囊对肾上腺素和去甲肾上腺素或神经刺激反应的增强作用的报道。前列腺素在男性生殖器官神经肌肉功能调节中的作用正在进一步研究中。虽然没有发现前列腺素（PG）在啮齿动物雄性副性器官中像在人类和公羊中那样以可识别的数量存在，但Shaw（1966）、Ramwell和Shaw（1966）观察到这些化合物在体外交感神经刺激下从大鼠附睾脂肪垫中释放出来。某些物种的主要来源可能在产生精液的器官以外的器官中，这些化合物可能被释放并通过自主兴奋转移到男性性器官，在那里它们可能在男性生殖过程中发挥真正的激素作用。本研究利用仓鼠精囊来确定几种前列腺素化合物对平滑肌的相对影响
Effects of prostaglandins E1, E2, A1, Fia on contractile responses of hamster seminal vesicle smooth muscles to stimulation by epinephrine, norepinephrine, and acetylcholine are compared. Hamster seminal vesicles provide suitable preparations for comparisons of physiological actions of these pharmacological agonists. Pairs of seminal vesicles from a single animal respond synchronously in the muscle bath preparation, and were used in these tests. Potentiation of the responses to these stimulants occurred in the presence of all the prostaglandins tested. The relative effectiveness of the potentiation was estimated on atg/ml. dose basis to approximate a 1:2:5:10 ratio for E1, E2, A1, and Fia, respec- tively, for epinephrine and norepinephrine. PGE2 was about as effective as PGE1 in poten- hating responses to acetylcholine stimulation. These results approximate and confirm those reported by Naimzada (1969) for potentiating actions of these PGs on the responses of guinea pig seminal vesicles to epinephrine and norepinephrine or to nerve stimulation. Further studies are in progress relating to the actions of prostaglandins in the regulation of neuromuscular function in male reproductive organs. Although prostaglandins (PG) have not been found to be present in rodent male accessory sex organs in identifiable quanti- ties as they have in humans and rams, Shaw (1966) and Ramwell and Shaw (1966) ob- served that these compounds are released from rat epididymal fat pads in vitro upon sympathetic nerve stimulation. Major sources in some speciesmay be in organs other than those producing seminal fluids, and these compounds might be released and transferred upon autonomic excitation to male sex organs where they could exert true hormonal effects of significance in male reproductive processes. Hamster seminal vesicleshave been utilizedin this study in an effort to determine the relative effects of several prostaglandin compounds on smooth