Microarray analysis of differentially expressed genes in cells resistant to HIV-1
Microarray analysis of differentially expressed genes in cells resistant to HIV-1
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DOI：
10.1016/j.imlet.2004.02.009
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发表时间：
2004-04-30
影响因子：
4.4
通讯作者：
Simm, M
中科院分区：
文献类型：
--
作者：
Kartvelishvili, A;Lesner, A;Simm, M
我们之前分离出了两个匹配的转化人类T细胞克隆：其中一个对HIV-1复制具有抵抗力并分泌HIV-1抵抗因子（HRF），第二个保留了亲本细胞系对HIV-1感染的易感性。我们采用 cDNA 阵列来研究与 HIV-1 耐药性的获得和 HRF 分泌相关的细胞基因表达变化谱。使用基于组织的免疫学/血液学阵列，我们鉴定了 HRF(+) 和 HRF(-) 细胞差异表达的 29 个转录本。 HRF(+)细胞表现出参与转录的II基因的选择性下调，其中一些基因与细胞对HIV-1的易感性或HIV-1转录本身的促进有关。在上调基因组中，有 3 个基因与细胞对 HIV-1 的抵抗力直接相关。放置在阵列上的 cDNA 之一，代表假设的蛋白质 KIAA0117，仅与源自 HRF(+) 细胞的聚 A(+) RNA 探针杂交。 RT-PCR 和 Northern 印迹证实了转录抑制子 (CTCF) 和假设蛋白 KIAA0117 这两个基因的特异性上调。 KIAA0117 转录物在抵抗 HIV-1 复制中的作用需要确定。 (C) 2004 Elsevier B.V. 保留所有权利。
We have previously isolated two matched transformed human T cell clones: one of which is resistant to HIV-1 replication and secretes an HIV-1 resistance factor(s) (HRF) and the second which retains the susceptibility of the parental cell line to HIV-1 infection. We employed cDNA arrays to investigate the spectrum of changes in cellular gene expression that correlate with the acquisition of HIV-1 resistance and the secretion of HRF Using a tissue based immunology/hematology array, we identified 29 transcripts that were differentially expressed by HRF(+) and HRF(-) cells. HRF(+) cells showed a selective down-regulation of I I genes involved in transcription, several of which are implicated in either susceptibility of cells to HIV-1 or the promotion of HIV-1 transcription itself. In the group of the up-regulated genes, three were linked directly to the cellular resistance to HIV-1. One of the cDNAs placed on the array, representing the hypothetical protein KIAA0117 hybridized only with poly A(+) RNA probes derived from HRF(+) cells. The specific up-regulation of two genes, the transcription repressor (CTCF) and hypothetical protein KIAA0117 was confirmed by RT-PCR and Northern blot. The role of KIAA0117 transcript in the resistance to HIV-1 replication needs to be determined. (C) 2004 Elsevier B.V. All rights reserved.