MOST HUMAN CARCINOMAS OF THE EXOCRINE PANCREAS CONTAIN MUTANT C-K-RAS GENES
MOST HUMAN CARCINOMAS OF THE EXOCRINE PANCREAS CONTAIN MUTANT C-K-RAS GENES
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DOI：
10.1016/0092-8674(88)90571-5
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发表时间：
1988-05-20
期刊：
影响因子：
64.5
通讯作者：
PERUCHO, M
中科院分区：
文献类型：
--
作者：
ALMOGUERA, C;SHIBATA, D;PERUCHO, M
应用聚合酶链反应（PCR）体外基因扩增和RNA酶A错配切割法检测胰腺癌组织中c-K-ras基因突变。我们使用冷冻的肿瘤标本和来自福尔马林固定的、石蜡包埋的肿瘤组织的单个5 μ m切片，所述肿瘤组织是手术切除的或尸检获得的。22例胰腺外分泌癌中有21例c-K-ras基因第12密码子突变。在7例受试病例中，该突变存在于原发性肿瘤及其相应的转移瘤中。在同一癌症患者的正常组织或5例胆囊癌中未检测到突变。我们从这些结果得出结论，c-K-ras体细胞突变激活是一个关键事件，在大多数，如果不是全部，人类胰腺外分泌癌的肿瘤发生。
Using in vitro gene amplification by the polymerase chain reaction (PCR) and mutation detecting by the RNAase A mismatch cleavage method, we have examined c-K-ras genes in human pancreatic carcinomas. We used frozen tumor specimens and single 5 .mu.m sections from formalin-fixed, paraffin-embedded tumor tissue surgically removed or obtained at autopsy. Twenty-one out of 22 carcinomas of the exocrine pancreas contained c-K-ras genes with mutations at codon 12. In seven cases tested, the mutation was present in both primary tumors and their corresponding metastases. No mutations were detected in normal tissue from the same cancer patients or in five gall bladder carcinomas. We conclude from these results that c-K-ras somatic mutational activation is a critical event in the oncogenesis of most, if not all, human cancers of the exocrine pancreas.