Induction of colitis-associated neoplasia in mice using azoxymethane and dextran sodium sulfate.
Induction of colitis-associated neoplasia in mice using azoxymethane and dextran sodium sulfate.
复制标题
使用氧化偶氮甲烷和葡聚糖硫酸钠诱导小鼠结肠炎相关肿瘤。
DOI：
10.1016/bs.mcb.2020.09.008
复制
发表时间：
2021
影响因子：
--
通讯作者：
Dannenberg,AndrewJ
中科院分区：
文献类型：
--
作者：
Montrose,DavidC;Makino,Tomoki;Basu,Srijani;Ito,Naotake;Dannenberg,AndrewJ
长期存在的炎症性肠病（IBD）增加了结直肠癌（CRC）发展的风险。这种增加在很大程度上是由于慢性肠道炎症使上皮暴露于致癌因子。此外，炎症发作后重复性伤口愈合事件与粘膜增殖增强相关，进一步增强了这种促肿瘤发生的环境。虽然已经确定了IBD发病机制及其相关肿瘤的多个因素，但还需要做更多的工作来开发和改进治疗方法，以改善疾病，从而降低结直肠癌的风险。小鼠模型已成为确定结肠炎相关肿瘤发病机制的相关因素和试验治疗的有用工具。这些方法包括化学诱导和基因工程方法，导致慢性炎症和肿瘤的发展。在这里，我们提出了一种循序渐进的方法，通过联合给药偶氮氧甲烷和右旋糖酐硫酸钠在小鼠中诱导炎症相关的结肠肿瘤。对该方法的详细描述将有助于其在科学界的应用，目的是进一步阐明结肠炎相关肿瘤发生的机制，并制定降低风险的干预措施。
Long-standing inflammatory bowel diseases (IBD) increase the risk for the development of colorectal cancer (CRC). This increase is due in large part to chronic intestinal inflammation which exposes the epithelium to pro-carcinogenic factors. Moreover, enhanced mucosal proliferation associated with repetitive wound healing events following an inflammatory episode, further enhance this pro-tumorigenic environment. Although multiple factors involved in IBD pathogenesis and its associated neoplasia have been identified, more work is needed to develop and improve therapies to ameliorate disease and thus reduce CRC risk. Murine models have served as useful tools to identify factors involved in the pathogenesis of colitis-associated neoplasia and test therapies. These include both chemically-induced and genetic engineering approaches, resulting in chronic inflammation and tumor development. Here, we present a step-by-step method of inducing inflammation-associated colon neoplasia by combining administration of azoxymethane and dextran sodium sulfate in mice. A detailed description of this methodology will facilitate its use in the scientific community with the goals of further elucidating the mechanisms underlying colitis-associated tumorigenesis and developing risk reducing interventions.