Activation of the inflammasome by amorphous silica and TiO2 nanoparticles in murine dendritic cells
Activation of the inflammasome by amorphous silica and TiO2 nanoparticles in murine dendritic cells
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DOI：
10.3109/17435390.2010.506957
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发表时间：
2011-09-01
期刊：
影响因子：
5
通讯作者：
Foerster, Irmgard
中科院分区：
文献类型：
--
作者：
Winter, Meike;Beer, Hans-Dietmar;Foerster, Irmgard
纳米材料越来越多地用于各种食品应用。特别是，纳米颗粒的无定形SiO2已经被包含在香料中。由于肠道树突状细胞（DC）可能是摄入颗粒的关键靶点，我们比较了非晶态二氧化硅纳米颗粒与细晶二氧化硅、微米级二氧化钛颗粒与纳米级二氧化钛颗粒对DC的体外影响。二氧化钛和二氧化钛纳米颗粒以及结晶二氧化硅导致DC上MHC-II、CD80和CD86的上调。此外，这些颗粒激活炎性小体，导致野生型（WT）小鼠显著分泌IL-1 β，而Caspase-1-或nlrp3缺陷小鼠不分泌IL-1 β。二氧化硅纳米粒子和结晶二氧化硅诱导细胞凋亡，而二氧化钛纳米粒子促进活性氧（ROS）的产生。由于无定形二氧化硅和TiO2纳米颗粒对DC的活化状态有很强的影响，我们建议对纳米颗粒作为食品添加剂进行体内和体外的深入研究，以确保其对消费者的安全性。
Nanomaterials are increasingly used in various food applications. In particular, nanoparticulate amorphous SiO2 is already contained, e. g., in spices. Since intestinal dendritic cells (DC) could be critical targets for ingested particles, we compared the in vitro effects of amorphous silica nanoparticles with fine crystalline silica, and micron-sized with nano-sized TiO2 particles on DC. TiO2- and SiO2-nanoparticles, as well as crystalline silica led to an upregulation of MHC-II, CD80, and CD86 on DC. Furthermore, these particles activated the inflammasome, leading to significant IL-1 beta-secretion in wild-type (WT) but not Caspase-1- or NLRP3-deficient mice. Silica nanoparticles and crystalline silica induced apoptosis, while TiO2 nanoparticles led to enhanced production of reactive oxygen species (ROS). Since amorphous silica and TiO2 nanoparticles had strong effects on the activation-status of DC, we suggest that nanoparticles, used as food additives, should be intensively studied in vitro and in vivo, to ensure their safety for the consumer.