Genome-wide analysis of long noncoding RNAs, microRNAs, and mRNAs forming a competing endogenous RNA network in repeated implantation failure
Genome-wide analysis of long noncoding RNAs, microRNAs, and mRNAs forming a competing endogenous RNA network in repeated implantation failure
复制标题
对反复植入失败中形成竞争性内源性 RNA 网络的长非编码 RNA、microRNA 和 mRNA 进行全基因组分析
DOI：
10.1016/j.gene.2019.144056
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发表时间：
2019-12-15
期刊：
影响因子：
3.5
通讯作者：
Zhang, Aijun
中科院分区：
文献类型：
--
作者：
Xu, Huihui;Zhou, Mingjuan;Zhang, Aijun
重复的植入衰竭（RIF）主要是由于子宫内膜接受度较差。长的非编码RNA（LNCRNA）可以调节子宫内膜接受能力并在竞争性内源性RNA（CERNA）理论中起作用。然而，lncRNA-mIRNA-mRNA网络在重复植入失败（RIF）中的调节机制尚不清楚。我们使用来自5名具有RIF的女性和5个对照组的RNA-Servisting获得了LNCRNA，mRNA和miRNA的RIF相关表达谱。共表达分析表明，三个功能模块富含免疫反应/炎症过程。在代谢/生物合成过程中富含两个功能模块，并将一个功能模块富含在细胞周期途径中。通过添加miRNA数据，重建了每个模块的CERNA调节关系。整个差异表达的RNA的CERNA网络揭示了10个HUB LNCRNA。其中分别参与了模块1，模块2和模块3。模块中的LNC00511和SLC26A4-AS1； H19在模块5中。 15个随机选择的RNA的实时聚合酶链反应（RT-PCR）与我们的测序数据一致。这些可以用作RIF的新型潜在生物标志物。此外，它们可能通过充当CERNA参与子宫内膜的接受度。
Repeated implantation failure (RIF) was mainly due to poor endometrium receptivity. Long noncoding RNAs (lncRNAs) could regulate endometrium receptivity and act in competing endogenous RNA (ceRNA) theory. However, the regulatory mechanism of the lncRNA-miRNA-mRNA network in repeated implantation failure (RIF) is unclear. We obtained RIF-related expression profiles of lncRNAs, mRNAs, and miRNAs using mid-secretory endometrial tissue samples from 5 women with RIF and 5 controls by RNA-sequencing. Co-expression analysis revealed that three functional modules were enriched in immune response/inflammation process; two functional modules were enriched in metabolic/biosynthetic process, and one functional module were enriched in cell cycle pathway. By adding the miRNA data, ceRNA regulatory relationship of each module was reconstructed. The ceRNA network of the whole differentially expressed RNAs revealed 10 hub lncRNAs. Among them, TRG-AS1, SIMM25, and NEAT1 were involved in the module1, module2, and module3, respectively; LNC00511 and SLC26A4-AS1 in the module4; H19 in the module5. The real-time polymerase chain reaction (RT-PCR) results of 15 randomly selected RNAs were consistent with our sequencing data. These can be used as novel potential biomarkers for RIF. Furthermore, they might be involved in endometrium receptivity by acting as ceRNA.