Identification of a Cathepsin B-like Protease in the Crevicular Fluid of Gingivitis Patients
Identification of a Cathepsin B-like Protease in the Crevicular Fluid of Gingivitis Patients
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牙龈炎患者龈沟液中组织蛋白酶 B 样蛋白酶的鉴定
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
J. Mcdonald
中科院分区：
文献类型：
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作者：
D. Eisenhauer;R. Hutchinson;T. Javed;J. Mcdonald
根据底物特异性、硫醇依赖性、最适 pH 值、动力学参数、pH 稳定性和抑制剂敏感性判断，牙龈炎患者的龈沟液含有显着水平的半胱氨酸蛋白酶，其特征为溶酶体蛋白酶组织蛋白酶 B。使用高灵敏度荧光测定程序确定 25 名牙龈炎患者的组织蛋白酶 B 活性平均水平。
Crevicular fluid from gingivitis patients contains significant levels of a cysteine protease which was characterized as the lysosomal protease cathepsin B, as judged by substrate specificity, thiol dependence, pH optimum, kinetic parameters, pH stability, and inhibitor sensitivities. A highly-sensitive fluorometric assay procedure was used to establish the mean level of cathepsin B activity for 25 gingivitis patients.